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ELISA Kit for 8-Hydroxydeoxyguanosine (8-OHdG)

ELISA Kit for 8-Hydroxydeoxyguanosine (8-OHdG)

Item no.	CEA660Ge-48T
Manufacturer	Cloud-Clone
Amount	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 26.81pg/mL
Citations	Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients.Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in RatsReduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of …Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population studyOxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infectionDieta hipercalórica e hipoxia intermitente crónica como modelos de obesidad y apnea obstructiva del sueño. Significado de la leptina en el control respiratorioThe Activity of Serum 8-Iso-Prostaglandin F2α as Oxidative Stress Marker in Patients with Diabetes Mellitus Type 2 and Associated Dyslipidemic HyperglycemiaOleuropein Ameliorates Cisplatin-induced Hematological Damages Via Restraining Oxidative Stress and DNA InjuryOxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-TemporalStudy: Oxidative Stress and Diabetic Salivary Glands.Oxidative Modification in the Salivary Glands of High Fat-Diet Induced Insulin Resistant Rats.Chronic high-protein diet induces oxidative stress and alters the salivary gland function in ratsOxidative modification of biomolecules in the non-stimulated and stimulated saliva of patients with morbid obesity treated with bariatric surgeryAntioxidant Defence, Oxidative Stress and Oxidative Damage in Saliva, Plasma and Erythrocytes of Dementia Patients. Can Salivary AGE be a Marker of Dementia?Chemokine Receptor CXCR4 Plays a Crucial Role in Mediating Oxidative StressInduced Podocyte InjuryMelatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in ratsProtective Effect of Origanum Oil on Alterations of Some Trace Elements and Antioxidant Levels Induced by Mercuric Chloride in Male RatsMicronuclei Formation and 8-Hydroxy-2-Deoxyguanosine EnzymeDetection in Ovarian Tissues After Radiofrequency Exposure at1800 MHz in Adult SpragueeDawley RatsRisk of glomerular filtration rate decline in patients with hypertrophic cardiomyopathy and obstructive sleep apnoeaSex differences in complex regional pain syndrome type I (CRPS-I) in miceMicronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult Sprague–Dawley RatsEffect of N-Acetylcysteine on Antioxidant Defense, Oxidative Modification, and Salivary Gland Function in a Rat Model of Insulin ResistanceExposure to Ti4Al4V Titanium Alloy Leads to Redox Abnormalities, Oxidative Stress, and Oxidative Damage in Patients Treated for Mandible FracturesOxidative DNA Damage and Carotid Intima Media Thickness as Predictors of Cardiovascular Disease in Prediabetic SubjectsAnti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspringRedox Balance, Antioxidant Defense, and Oxidative Damage in the Hypothalamus and Cerebral Cortex of Rats with High Fat Diet-Induced Insulin ResistanceJournal of Pharmacological ReportsCross-sectional correlates of oxidative stress and inflammation with glucose intolerance in prediabetesOral Administration of Lactobacillus delbrueckii during the Suckling Phase Improves Antioxidant Activities and Immune Responses after the Weaning Event in a Piglet …Luteolin Attenuates Cardiac Ischemia/Reperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative FunctionDendrobium Officinale Polysaccharides Protect against MNNG-Induced PLGC in Rats via Activating the NRF2 and Antioxidant Enzymes HO-1 and NQO-1Prophylactic Impact of Beta vulgaris L in Ameliorating Cyclosporine A-Induced Hepatotoxicity in Rats.Assessment of 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as a Diagnostic Marker for Diabetic Nephropathy in Type 1 Egyptian Diabetic PatientsDietary supplementation of barley and/or dates attenuate hypercholesterolemic‐induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic …Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brainsLeukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese PopulationDysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese AdolescentsHydrogen Sulfide Attenuates Particulate Matter-Induced Emphysema and Airway Inflammation Through Nrf2-Dependent MannerSalivary 8-hydroxyguanosine levels in smokers and non-smokers with chronic periodontitisNegative association between antioxidant vitamin intake and non-alcoholic fatty liver disease in Chinese non-diabetic adults: mediation models involving superoxide …Lycopene protects neuroblastoma cells against oxidative damage via depression of ER stressAlteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot studyTriglyceride is independently correlated with insulin resistance and islet beta cell function: a study in population with different glucose and lipid metabolism …Rescuing Dicer expression in inflamed colon tissues alleviates colitis and prevents colitis-associated tumorigenesisSystemic Redox Imbalance in Patients with Chronic Granulomatous DiseaseNAC Supplementation of Hyperglycemic Rats Prevents the Development of Insulin Resistance and Improves Antioxidant Status but Only Alleviates General …Potential Protective Effect of Dietary Intake of Non-α-Tocopherols on Cellular Aging Markers Mediated by Tumor Necrosis Factor-α in Prediabetes: A Cross …Relationship between Decreased Serum Superoxide Dismutase Activity and Metabolic Syndrome: Synergistic Mediating Role of Insulin Resistance and β …Induction of genotoxicity and mutagenic potential of heavy metals in Thai occupational workersOral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in pigletsChanges and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from ChinaThe potential protective role of gamma-irradiated vaccine versus Punica granatum treatment against murine trichinellosisMutat Res Gen Tox EnElectroacupuncture ameliorates motor dysfunction via inhibiting p66Shc-mediated oxidative stress and endoplasmic reticulum stress in rats with spinal cord …8-OHdG and hOGG1 as Oxidative DNA Damage Markers in Acne Vulgaris Patients Under Isotretinoin TreatmentBiomarkers of nucleic acid oxidation¨Ca summary state-of-the-artAntioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damageNaringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic ratsOxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspectiveSestrin2 is involved in Nrf2-regulated antioxidative signaling pathway in luteolin-induced prevention of the diabetic rat heart from ischemia/reperfusion injuryGrowth performance, antioxidant capacity, tissue fatty acid composition and lipid metabolism of juvenile green mud crab Scylla paramamosain in response to?¡Role of Yoga and Its Plausible Mechanism in the Mitigation of DNA Damage in Type-2 Diabetes: A Randomized Clinical TrialAssociation of Tumour Microenvironment with Protein Glycooxidation, DNA Damage, and Nitrosative Stress in Colorectal CancerHigh Hemoglobin glycation index is associated with telomere attrition independent of HbA1c, mediating by TNFαChromium induced neurotoxicity by altering metabolism in zebrafish larvaeEvaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic NephropathyBody mass index is associated with Helicobacter pylori infection and increased oxidative DNA damage in an obese populationBiochemical and Molecular Studies of Different Parameters as Markers for Nephropathy in Type 1 Diabetic Patients
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	8OHdG,7,8-Dihydro-8-Oxo-2'-Deoxyguanosine,7,8-Dihydro-8-Oxodeoxyguanosine,8-Hydroxy-2'-Deoxyguanosine,8-Oxo-DG
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	Serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of 8-Hydroxydeoxyguanosine (8-OHdG). No significant cross-reactivity or interference between 8-Hydroxydeoxyguanosine (8-OHdG) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 8-Hydroxydeoxyguanosine (8-OHdG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 8-Hydroxydeoxyguanosine (8-OHdG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 8-Hydroxydeoxyguanosine (8-OHdG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 8-Hydroxydeoxyguanosine (8-OHdG) and unlabeled 8-Hydroxydeoxyguanosine (8-OHdG) (Standards or samples) with the pre-coated antibody specific to 8-Hydroxydeoxyguanosine (8-OHdG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 8-Hydroxydeoxyguanosine (8-OHdG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 8-Hydroxydeoxyguanosine (8-OHdG) in the sample.
Manufacturer - Research Area	Metabolic pathway; Tumor immunity; Infection immunity; Endocrinology; Hematology; Hepatology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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