« Back
Home /
ELISA Kit for Follicle Stimulating Hormone (FSH)

ELISA Kit for Follicle Stimulating Hormone (FSH)

Item no.	CEA830Ra-96T
Manufacturer	Cloud-Clone
Amount	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Testicular & Prostate Cancer Uterine, Ovarian & Cervical Cancer
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.94ng/mL
Citations	Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats Central effects of camphor on GnRH and sexual hormones in male rat Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats The effect of R-(-)-deprenyl administration on reproductive parameters of rat males Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats Sex-specific prenatal stress effects on the rat reproductive axis and adrenal gland structure Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats. Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. Impact of uteroplacental insufficiency on postnatal rat male gonad. Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) … Palladium nanoparticle effects on endocrine reproductive system of female rats Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide … The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study. Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance … MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS Hemin Ameliorated Letrozole-Induced Polycystic Ovary Syndrome: A Forward Step for Better Management Drug-Herb Interaction Between Selective Estrogen Receptor Modulators and Estrogenic Herbal Medicine Er-Xian Decoction in Bone in Vivo and in Vitro Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis The Effects of Zinc Methionine on Reproductive and Thyroid Hormones in Rats with Polycystic Ovarian Syndrome Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment Water extract of Er-xian decoction selectively exerts estrogenic activities and interacts with SERMs in estrogen-sensitive tissues Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency Endothelial dysfunction after androgen deprivation therapy and the possible underlying mechanisms Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with … G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and … Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency A promising animal model for Polycystic Ovary Syndrome: Borderline Hypertensive Rats
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Follicle Stimulating Hormone (FSH). No significant cross-reactivity or interference between Follicle Stimulating Hormone (FSH) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Follicle Stimulating Hormone (FSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Follicle Stimulating Hormone (FSH) and unlabeled Follicle Stimulating Hormone (FSH) (Standards or samples) with the pre-coated antibody specific to Follicle Stimulating Hormone (FSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample.
Manufacturer - Research Area	Endocrinology; Reproductive science; Genetic science; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.