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ELISA Kit for Dopamine (DA)

ELISA Kit for Dopamine (DA)

Item no.	CEA851Ge-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Metabolism Diabetes ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bone Cancer
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 4.71pg/mL
Citations	Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults Nalbuphine could decrease the rewarding effect induced by tramadol in mice while enhancing its antinociceptive activity Catha edulis chewing effects on treatment of paranoid schizophrenic patients Neuroprotective effects of vildagliptin in rat rotenone Parkinson' s disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathways Modulatory effect of cilostazol on tramadol-induced behavioral and neurochemical alterations in rats challenged across the forced swim despair test Imipramine and amitriptyline ameliorate the rotenone model of Parkinson's disease in rats Neuroprotective effect of Cucumis melo Var. flexuosus leaf extract on the brains of rats with streptozotocin-induced diabetes. Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats High Fat Diet feeding induced insulin resistance exacerbates 6-OHDA mediated neurotoxicity and behavioral abnormalities in rats Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats Dopamine levels in human tear fluid Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles Soya bean rich diet is associated with adult male rat aggressive behavior: relation to RF amide-related peptide 3-aromatase-neuroestrogen pathway in the brain Functional activity of p-glycoprotein in blood-brain barrier during experimental par-kinson's syndrome Neurotoxicity of Mn3O4 nanoparticles: Apoptosis and dopaminergic neurons damage pathway Polydatin protects SH-SY5Y in models of Parkinson's disease by promoting Atg5-mediated but parkin-independent autophagy Iron overload induced by IRP2 gene knockout aggravates symptoms of Parkinson's disease Neurotransmitter system aberrations in patients with drug addiction Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemia Effect of metformin and indole-3-carbinol on a rat model of Parkinson's disease induced by 6-hydroxydopamine Neuroprotective effects of pulicaria undulata essential oil in rotenone model of parkinson's disease in rats: Insights into its anti-inflammatory and anti-oxidant effects The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6?¡ Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡ O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress A pair of dopamine neurons mediate chronic stress signals to induce learning deficit in Drosophila melanogaster Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson's Disease Effects of early life stress during stress hyporesponsive period (SHRP) on anxiety and curiosity in adolescent rats
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	2-(3,4-Dihydroxyphenyl)ethylamine,3,4-Dihydroxyphenethylamine,3-Hydroxytyramine,Intropin,Revivan,Oxytyramine,Dopamine Hydrochloride
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	Serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Dopamine (DA). No significant cross-reactivity or interference between Dopamine (DA) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine (DA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine (DA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Dopamine (DA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Dopamine (DA) and unlabeled Dopamine (DA) (Standards or samples) with the pre-coated antibody specific to Dopamine (DA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Dopamine (DA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Dopamine (DA) in the sample.
Manufacturer - Research Area	Signal transduction; Cardiovascular biology; Reproductive science; Neuro science;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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