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ELISA Kit for Calcitonin Gene Related Peptide (CGRP)

ELISA Kit for Calcitonin Gene Related Peptide (CGRP)

Item no.	CEA876Hu-96T
Manufacturer	Cloud-Clone
Amount	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Molecular Targets for Neuroscience Cell Biology Cell Status Necrosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Thyroid Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 2.23pg/mL
Citations	Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo.Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological markerCGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic MigrainePrognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot.Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control studyOnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migraineEffect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitisRelationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patientsTransient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction.Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel SyndromeFeasibility of serum CGRP measurement as a biomarker of chronic migraine: a critical reappraisalPeriodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migraineElectrical stimulation of the superior sagittal sinus suppresses A-type K currents and increases P/Q-and T-type Ca 2 currents in rat trigeminal ganglion …Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headacheSerum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practiceMild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineursPerirenal adipose afferent nerves sustain pathological high blood pressure in rats
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CALCB,CGRP2,CALC2,CGRP-II,Calcitonin Gene Related Peptide 2,Calcitonin 2,Calcitonin Related Polypeptide Beta
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP). No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Calcitonin Gene Related Peptide (CGRP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Calcitonin Gene Related Peptide (CGRP) and unlabeled Calcitonin Gene Related Peptide (CGRP) (Standards or samples) with the pre-coated antibody specific to Calcitonin Gene Related Peptide (CGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample.
Manufacturer - Research Area	Infection immunity; Endocrinology; Neuro science; Hormone metabolism; Bone metabolism; Dermatology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.