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ELISA Kit for Gamma-Aminobutyric Acid (gABA)

ELISA Kit for Gamma-Aminobutyric Acid (gABA)

Item no.	CEA900Ge-24T
Manufacturer	Cloud-Clone
Amount	24 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	ELISA & Immunoassays ELISA Kits
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 2.17pg/mL
Citations	Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellumDecreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with AutismCorrelation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic ChildrenDecreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced HypertensionSex differences in the glutamate signaling pathway in juvenile ratsA novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzymeAucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of NeurotransmissionDifferentiation of human glioblastoma U87 cells into cholinergic neuronGlutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of AutismEffect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsyDementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantationAge-dependent neurological phenotypes in a mouse model of PRRT2-related diseasesOverexpression of wild-type human amyloid precursor protein alters GABAergic transmissionAdaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	4-Aminobutyric Acid
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Gamma-Aminobutyric Acid (gABA). No significant cross-reactivity or interference between Gamma-Aminobutyric Acid (gABA) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gamma-Aminobutyric Acid (gABA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gamma-Aminobutyric Acid (gABA) and unlabeled Gamma-Aminobutyric Acid (gABA) (Standards or samples) with the pre-coated antibody specific to Gamma-Aminobutyric Acid (gABA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gamma-Aminobutyric Acid (gABA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gamma-Aminobutyric Acid (gABA) in the sample.
Manufacturer - Research Area	Signal transduction; Cardiovascular biology; Neuro science; Bone metabolism

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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