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ELISA Kit for Noradrenaline (NE)

ELISA Kit for Noradrenaline (NE)

Item no.	CEA907Ge-96T
Manufacturer	Cloud-Clone
Amount	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Food Safety Hormones Neuroscience Metabolism Glucose Homeostasis Molecular Targets for Neuroscience Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 23.5pg/mL
Citations	Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients Apelin-13 and APJ in paraventricular nucleus contribute to hypertension via sympathetic activation and vasopressin release in spontaneously hypertensive rats Renal Sympathetic Denervation Improves Cardiac Dysfunction Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of 伪1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta. Catha edulis chewing effects on treatment of paranoid schizophrenic patients Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of α1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta Relaxin in paraventricular nucleus contributes to sympathetic overdrive and hypertension via PI3K-Akt pathway Determination of 17β-estradiol and noradrenaline in dog serum using surface-enhanced Raman spectroscopy and random Forest Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity Artepillin C, a Typical Brazilian Propolis-Derived Component, Induces Brown-Like Adipocyte Formation in C3H10T1/2 Cells, Primary Inguinal White Adipose Tissue-Derived Adipocytes, and Mice. Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats Effects of renal denervation on monocrotaline induced pulmonary remodeling Renal sympathetic denervation attenuates hypertension and vascular remodeling in renovascular hypertensive rats Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial A novel sympathetic neuronal GABA ergic signaling system regulates NE release to prevent ventricular arrhythmias after acute myocardial infarction Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles Noninvasive Stereotactic Radiotherapy for Renal Denervation in a Swine Model Exacerbated pressor and sympatho-excitatory effects of central Elabela in spontaneously hypertensive rats Resiniferatoxin reduces ventricular arrhythmias in heart failure via selectively blunting cardiac sympathetic afferent projection into spinal cord in rats A porcine model for studying the cardiovascular consequences of high‐thoracic spinal cord injury Role of melatonin in intestinal mucosal injury induced by restraint stress in mice Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Laparoscopic-based perivascular renal sympathetic nerve denervation: a feasibility study in a porcine model Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammation Baroreceptor denervation reduces inflammatory status and worsens cardiovascular collapse during systemic inflammation Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair … Fractionation and bioassay-guided isolation of antihypertensive components of senecio serratuloides The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡ The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models Renal interstitial fibrosis is reduced in high-fat diet-induced obese pigs following renal denervation from the intima and adventitia of the renal artery
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	NA,NAd,Norepinephrine,Norepi
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Noradrenaline (NE). No significant cross-reactivity or interference between Noradrenaline (NE) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Noradrenaline (NE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Noradrenaline (NE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Noradrenaline (NE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Noradrenaline (NE) and unlabeled Noradrenaline (NE) (Standards or samples) with the pre-coated antibody specific to Noradrenaline (NE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Noradrenaline (NE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Noradrenaline (NE) in the sample.
Manufacturer - Research Area	Signal transduction; Infection immunity; Neuro science; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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