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ELISA Kit for Histamine (HA)

ELISA Kit for Histamine (HA)

Item no.	CEA927Ge-48T
Manufacturer	Cloud-Clone
Amount	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Food Safety Allergenes Histamines Metabolism Cholesterol & Lipid Metabolism ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	other
Sensitivity	The minimum detectable dose of this kit is typically less than 137.3pg/mL
Citations	PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in mice Comparison of sensitization between β-lactoglobulin and its hydrolysates Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea Induction of immune responses and allergic reactions in piglets by injecting glycinin Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbumin Evaluation of anaphylactoid constituents in vitro and in vivo. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy CCR3‑shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Histamin
Available
Specificity	Pan-species (General)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Manufacturer - Species	Pan-species (General)
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Histamine (HA). No significant cross-reactivity or interference between Histamine (HA) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histamine (HA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histamine (HA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Histamine (HA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Histamine (HA) and unlabeled Histamine (HA) (Standards or samples) with the pre-coated antibody specific to Histamine (HA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Histamine (HA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Histamine (HA) in the sample.
Manufacturer - Research Area	Signal transduction; Infection immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.