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ELISA Kit for Hepcidin (Hepc)

ELISA Kit for Hepcidin (Hepc)

Item no.	CEB979Hu-96T
Manufacturer	Cloud-Clone
Amount	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Neural Transcription Factors ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Testicular & Prostate Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 11pg/mL
Citations	Serum iron metabolism markers including hepcidin in multiple sclerosis patients Monitoring Hepcidin Level in Chronic Hepatitis C Virus Patients during Therapy Hepcidin and regulation of iron homeostasis in maintenance hemodialysis patients The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders An Evaluation of the Correlation between Hepcidin Serum Levels and Disease Activity in Inflammatory Bowel Disease Serum hepcidin levels in Bulgarian population. Liver Congestion in Heart Failure Contributes to Inappropriately Increased Serum Hepcidin despite Anemia Hepcidin regulation in prostate and its disruption in prostate cancer Effect of a More Permeable Dialysis Membrane on ESA Resistance in Hemodialysis Patients - A Pilot Investigation Urinary Hepcidin in Diagnosis of Iron Deficiency Anemia Hepcidin in tumor‐related iron deficiency anemia and tumor‐related anemia of chronic disease: pathogenic mechanisms and diagnosis The Effects of Treatment on Serum Hepcidin and Iron Homeostasis in HIV-1-Infected In-dividuals Hydrogen sulfide attenuates inflammatory hepcidin by reducing IL-6 secretion and promoting SIRT1-mediated STAT3 deacetylation Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients AMPK serves as a Therapeutic Target against Anemia of Inflammation Hepcidin suppression in β‑thalassemia is associated with the down‑regulation of atonal homolog 8 Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. Increased NGAL level associated with iron store in chronic kidney disease with anemia Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? Hepcidin serum levels in HCV chronically mono-infected naïve patients, compared to healthy individuals Increased Hepcidin Levels During a Period of High Training Load Do Not Alter Iron Status in Male Elite Junior Rowers Can Calprotectin Show Subclinical Inflammation in Familial Mediterranean Fever Patients? Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in … Urinary hepcidin concentration in assessment of iron homeostasis in pediatrics COMPARATIVE MONITORING OF THE ENZYME ACTIVITY OF BONE METABOLISM IN PATIENTS AFTER DENTAL IMPLANTATION Serum iron, prealbumin, D-dimer and prothrombin time are indicators of the diagnosis, severity and outcome of Salmonella enterica serovar Enteritidis infection Гепсидин и индекс фиброза печени MELD-XI как маркеры полиорганной недостаточности у пациентов хронической сердечной недостаточностью с … Ассоциации уровней NT-proBNP и гепсидина с клинико-лабораторными параметрами у больных хронической сердечной недостаточностью с разной … Correlation of hepcidin and serum ferritin levels in thalassemia patients at Chiang Mai University Hospital Continuous Veno-Venous Hemofiltration (CVVH) Improves Iron Metabolism Disorders in Patients With Sepsis: A Single-Center Prospective Cohort Study Evaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization Caffeine and alcohol-friends or foes of human iron stores? Serum Calprotectin and Iron Metabolism Biomarker Levels in Behçet's Disease
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	HAMP,HFE2B,PLTR,LEAP1,Hepcidin Antimicrobial Peptide,Liver-expressed antimicrobial peptide 1,Putative liver tumor regressor
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc). No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hepcidin (Hepc) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hepcidin (Hepc) and unlabeled Hepcidin (Hepc) (Standards or samples) with the pre-coated antibody specific to Hepcidin (Hepc). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hepcidin (Hepc) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hepcidin (Hepc) in the sample.
Manufacturer - Research Area	Metabolic pathway; Endocrinology; Hematology; Genetic science;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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