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ELISA Kit for Salusin Beta (SALb)

ELISA Kit for Salusin Beta (SALb)

Item no.	CEC026Hu-5x96T
Manufacturer	Cloud-Clone
Amount	5x96 Tests
Quantity options	10x96 Tests 24 Tests 48 Tests 5x96 Tests 96 Tests
Category	Oncology Food Safety Allergenes Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Uterine, Ovarian & Cervical Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 9.77pg/mL
Citations	Serum levels of apelin, salusin-alpha, and salusin-beta in normal pregnancy and preeclampsiaThe bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypassPresence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cowsAssociation of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses.Serum concentration and kidney expression of salusin-α and salusin-β in rats with metabolic syndrome induced by fructose.Salusins, newly identified regulators of hemodynamics and mitogenesis, increase in polycystic ovarian syndrome.Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitusSalusin-alpha and -beta expression in heart and aorta with and without metabolic syndromeBrain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic SyndromeIs the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population?Serum salusin-α and salusin-β levels in patients with psoriasisSerum Salusin-β Levels Are Associated With the Presence and Severity of Coronary Artery DiseaseSerum Salusin-β Levels Are Correlated with Slow Coronary Flow.Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathyIncreased level of Plasma Salusin-α and Salusin-β in patients with Multiple SclerosisSalusin-α levels are negatively correlated with diastolic blood pressure in children with obesitySalusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosisSerum level of salusin β as an indicator of metabolic disorders in Acute Lymphoblastic Leucaemia and Wilms Tumour survivorsSalusin‑β participates in high glucose‑induced HK‑2 cell ferroptosis in a Nrf‑2‑dependent manner
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma and other biological fluids
Assay length	2h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Salusin Beta (SALb). No significant cross-reactivity or interference between Salusin Beta (SALb) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Salusin Beta (SALb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Salusin Beta (SALb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50uL Stop Solution. Read at 450 nm immediately.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Salusin Beta (SALb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Salusin Beta (SALb) and unlabeled Salusin Beta (SALb) (Standards or samples) with the pre-coated antibody specific to Salusin Beta (SALb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Salusin Beta (SALb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Salusin Beta (SALb) in the sample.
Manufacturer - Research Area	Metabolic pathway; Endocrinology; Cardiovascular biology; Neuro science; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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