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Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP)

Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP)

Item no.	MEA882Ra-24T
Manufacturer	Cloud-Clone
Amount	24 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	ELISA & Immunoassays ELISA Kits
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 21.9pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Incretin hormone; Glucose Dependent Insulinotropic Peptide
Available
Specificity	Rattus norvegicus (Rat)
Quantity options
Detection range	61.7-5, 000pg/mL
Organism species	Rattus norvegicus (Rat)
Sensitivity	The minimum detectable dose of this kit is typically less than 21.9pg/mL
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Mini Samples Gastric Inhibitory Polypeptide (GIP). No significant cross-reactivity or interference between Mini Samples Gastric Inhibitory Polypeptide (GIP) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Gastric Inhibitory Polypeptide (GIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Gastric Inhibitory Polypeptide (GIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Gastric Inhibitory Polypeptide (GIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Gastric Inhibitory Polypeptide (GIP) and unlabeled Mini Samples Gastric Inhibitory Polypeptide (GIP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Gastric Inhibitory Polypeptide (GIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Gastric Inhibitory Polypeptide (GIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Gastric Inhibitory Polypeptide (GIP) in the sample.
Research Area	Endocrinology; Gastroenterology; Hormone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.