IL-27 p28 ELISA Kit (Mouse)

ELISA Kit

Wet Ice

interleukin 27

Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. By genomic sequence analysis, the IL27 p28 gene was mapped to chromosome 16p11. L-27 plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130.

NP_663611.1

Expression system for standard: NS0; Immunogen sequence: F29-S234

Assay Methodology: Quantitative Sandwich Immunoassay

Aviva's mouse IL-27 p28 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-27 p28 has been precoated onto 96-well plates. Standards(NS0, F29-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-27 p28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL-27 p28 amount of sample captured in plate.