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ELISA Kit for Interferon Gamma (IFNg)

ELISA Kit for Interferon Gamma (IFNg)

Item no.	SEA049Bo-24T
Manufacturer	Cloud-Clone
Amount	24 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	ELISA & Immunoassays ELISA Kits
Type	Elisa-Kit
Specific against	Cow (Bos taurus), Cattle (Bovine)
Sensitivity	The minimum detectable dose of this kit is typically less than 5.7pg/mL
Citations	Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in?Escherichia coli?induce potent protective immune responses in guinea pigs, swine and cattleEvaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cellsCOMPARATIVE EFFICACY OF TWO HETEROLOGOUS CAPRIPOX VACCINES TO CONTROL LUMPY SKIN DISEASE IN CATTLEEvaluation of Cell-mediated Immune Response in PBMCs of Calves Vaccinated by Capri Pox Vaccines Using ELISA and Real-time RT-PCREffect of reproductive disorders on productivity and reproductive efficiency of dromedary she-camels in relation to cytokine concentrationImmune response characteristics of Capri pox virus vaccines following emergency vaccination of cattle against lumpy skin disease virusPD-1 blockade inhibits lymphocyte apoptosis and restores proliferation and anti-viral immune functions of lymphocyte after CP and NCP BVDV infection in vitroEarly cytokine response after vaccination with Coxiella burnetii phase I in an infected herd of dairy cattleEffect of postpartum administration of ketoprofen on proinflammatory cytokine concentration and their correlation with lipogenesis and ketogenesis in Holstein …Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …Association Between Serum Selenium Level and Subclinical Mastitis in Dairy CattleHormonal and Cytokine Blood Profile of Dairy Cows in the Early Gestation PeriodEffect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calvesHow Does the Th1/Th2 Polarization in Milk Change According to the Types of Bacteria that Cause Subclinical Mastitis in Cows?Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	IFN-G,IFG,IFI,INFr,IFN,Immune Interferon
Available
Specificity	Bos taurus; Bovine (Cattle)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interferon Gamma (IFNg). No significant cross-reactivity or interference between Interferon Gamma (IFNg) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma (IFNg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Gamma (IFNg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma (IFNg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Gamma (IFNg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Infection immunity

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.