« Back
Home /
ELISA Kit for Interferon Gamma (IFNg)

ELISA Kit for Interferon Gamma (IFNg)

Item no.	SEA049Hu-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Neural Transcription Factors Aging & Neurological Disorders Infectious Diseases Viral Infections Coronavirus (COVID-19) Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Heart & Blood Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 6.1pg/mL
Citations	The immunomodulating effect of seminal plasma on T cellsNanogel‐Incorporated Physical and Chemical Hybrid Gels for Highly Effective Chemo–Protein Combination TherapyRituximab-induced HMGB1 release is associated with inhibition of STAT3 activity in human diffuse large B-cell lymphomaGemcitabine treatment enhanced the anti-tumor effect of cytokine induced killer cells by depletion of CD4+CD25bri regulatory T cells.The effects of Spirulina platensis (Arthrospira platensis) and Saccharomyces cerevisiae on the distribution and cytokine production of CD4+ and CD8+ T …Evaluation of Cell-mediated Immune Response in PBMCs of Calves Vaccinated by Capri Pox Vaccines Using ELISA and Real-time RT-PCRCombination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cellsAssociations among interleukin-17, interferon-gamma, and acute coronary syndromeIdentification and preliminary study of immunogens involved in autoimmune prostatitis in human malesAntitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitroIncreased Macroautophagy in Interferon-Gamma-Producing T Cells from Patients with Newly Diagnosed Systemic Lupus ErythematosusChanges in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary diseaseخصوصیات پاسخ‌های ایمنی القاء شده ناشی از واکسن زنده تخفیف حدت یافته آبله بزی سویه گرگان بر علیه بیماری لمپی اسکین در گاو‎Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in …Construction and Immunogenicity of Novel Chimeric Virus-Like Particles Bearing Antigens of Infectious Bronchitis Virus and Newcastle Disease VirusDisseminated And Infection Accompanied Sweet's Syndrome In A Patient With Anti-Interferon-γ Autoantibodies: A Case Report.Serum sHLA-G: Significant diagnostic biomarker with respect to therapy and immunosuppressive mediators in Head and Neck Squamous Cell CarcinomaPIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escapeDecreased suppression of CD8+ and CD4+ T cells by peripheral Regulatory T cells in Generalized Vitiligo due to reduced NFATC1 and FOXP3 proteinsTIM-3 blockade combined with bispecific antibody MT110 enhances the anti-tumor effect of γδ T cellsSalivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patientsTalaromyces marneffei and Burkholderia cepacia Co-Infection in a HIV-Uninfected Patient with Anti-Interferon-¦Ã AutoantibodiesMelatonin-mediated MT2 attenuates colitis induced by dextran sodium sulfate via PI3K/AKT/Nrf2/SIRT1/ROR¦Á/NF-¦ÊB signaling pathwaysAmplified Antitumor Efficacy By A Targeted Drugs Retention and Chemosensitization Strategies-Based ¡°Combo¡± Nanoagent Together With PD-L1 Blockade In?¡Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axisPDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patientsNovel interferon-sensitive genes unveiled by correlation-driven gene selection and systems biologyAssociations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive …Retrospective Analysis of 10 Cases of Disseminated Nontuberculous Mycobacterial Disease with Osteolytic LesionsIn-vitro oxidative potential and inflammatory response of ambient PM2. 5 in a rural region of Northwest China: Association with chemical compositions and …
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	IFN-G,IFG,IFI,INFr,IFN,Immune Interferon
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interferon Gamma (IFNg). No significant cross-reactivity or interference between Interferon Gamma (IFNg) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma (IFNg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Gamma (IFNg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma (IFNg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Gamma (IFNg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Infection immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.