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ELISA Kit for Interleukin 10 (IL10)

ELISA Kit for Interleukin 10 (IL10)

Item no.	SEA056Mu-5x96T
Manufacturer	Cloud-Clone
Amount	5x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Food Safety Allergenes Neuroscience Neuroinflammation Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Skin Cancer Lymphatic System Cancer
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	The minimum detectable dose of this kit is typically less than 2.9pg/mL
Citations	Optimal formation of genetically modified and functional pancreatic islet spheroids by using hanging-drop strategyEfficacy of Albendazole-Chitosan Microsphere-based Treatment for Alveolar Echinococcosis in MiceTrypanosoma cruzi infection and benznidazole therapy independently stimulate oxidative status and structural pathological remodeling of the liver tissue in miceHypoxia-induced secretion of IL-19 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaModulation of oxidative and inflammatory cardiac response by nonselective 1‐and 5‐cyclooxygenase inhibitor and benznidazole in miceA chimeric protein composed of the binding domains ofClostridium perfringens phospholipase C and Trueperella pyogenes pyolysin induces partial immunoprotection in a mouse modelLipopolysaccharide Cross-Tolerance Delays Platelet-Activating Factor-Induced Sudden Death in Swiss Albino Mice: Involvement of Cyclooxygenase in Cross-ToleranceInfluences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiationNonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease in T. cruzi-infected mice.Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in miceEfeito protetor do β-glucano e glutamina em lesões intestinais e imunológicas induzidas por ciratabina (Ara-C) em camundongosBuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathwayA Systems Pharmacology Approach to Determine Active Compounds and Action Mechanisms of Xipayi KuiJie’an enema for Treatment ofUlcerative colitisOral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in miceRole of 3-acetyl-11-keto-beta-boswellic acid in counteracting LPS-induced neuroinflammation via modulation of miRNA-155Effects of peritoneal macrophage autophagy on the immune function of sepsis mice.Grape Seed Procyanidin Extract Reduces Arsenic-Induced Renal Inflammatory Injury in Male Mice.Nose-to-brain delivery of insulin enhanced by a nanogel carrierSonodynamic therapy‐assisted immunotherapy: A novel modality for cancer treatmentTargeting central β2 receptors ameliorates streptozotocin-induced neuroinflammation via inhibition of glycogen synthase kinase3 pathway in miceResolution of bleomycin-induced murine pulmonary fibrosis via a splenic lymphocyte subpopulationChitosan oligosaccharide ameliorates acute lung injury induced by blast injury through the DDAH1/ADMA pathwayA Novel scFv Anti-Aβ Antibody Reduces Pathological Impairments in APP/PS1 Transgenic Mice via Modulation of Inflammatory Cytokines and Aβ-related EnzymesShock waves increase pulmonary vascular leakage, inflammation, oxidative stress, and apoptosis in a mouse modelLipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axisBushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling …Natural killer T cell ligand alpha-galactosylceramide protects against gut ischemia reperfusion-induced organ injury in miceThe relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis miceDNA prime‐protein boost vaccine encoding HLA‐A2,‐A24 and‐DR 1 restricted epitopes of Ca NA 2 against visceral leishmaniasisRecombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathwayEnhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic …Ovotransferrin ameliorates the dysbiosis of immunomodulatory function and intestinal microbiota induced by cyclophosphamideThree types of gut bacteria collaborating to improve Kui Jie'an enema treat DSS-induced colitis in miceChlorogenic Acid Attenuates Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice through MAPK/ERK/JNK PathwayNeuroinflammation induced by lipopolysaccharide causes cognitive impairment in miceRole of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in micePhotothermal-triggered Controlled Drug Release From Mesoporous Silica nanoparticles Based On Base-pairing RulesCD28 deficiency attenuates primary blast-induced renal injury in mice via the PI3K/Akt signalling pathwayAdenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in miceMODULATION OF LIPOPOLYSACCHARIDE-INDUCED CYTOKINES RESPONSE IN MICE BY GRAVIOLA LEAF POWDERPolysaccharide from the Seeds of Plantago asiatica L. Protect Against Lipopolysaccharide-Induced Liver InjuryEnhancement of the therapeutic efficacy of praziquantel in murine Schistosomiasis mansoni using silica nanocarrierMelatonin alleviates oxidative stress in sleep deprived mice: Involvement of small intestinal mucosa injuryThe Increase in IL-1β in the Early Stage of Heatstroke Might Be Caused by Splenic Lymphocyte Pyroptosis Induced by mtROS-Mediated Activation of the …The toxicity of alumina nanoparticles to immune system in miceSuppression of Fpr2 expression protects against endotoxin-induced acute lung injury by interacting with Nrf2-regulated TAK1 activationCalycosin-7-O-β-d-glucoside attenuates myocardial ischemia–reperfusion injury by activating JAK2/STAT3 signaling pathway via the regulation of IL-10 secretion in …STAT5a induces endotoxin tolerance by alleviating pyroptosis in kupffer cellsPorcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasionImpact of diminazene aceturate on renin-angiotensin system, infectious myocarditis and skeletal myositis in mice: An in vitro and in vivo studyRecombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF-κB pathwayUSP8 protects against lipopolysaccharide-induced cognitive and motor deficits by modulating microglia phenotypes through TLR4/MyD88/NF-κB signaling pathway in …M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in MiceNicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial …Protective Effects of Carbon Dots Derived from Phellodendri Chinensis Cortex Carbonisata against Deinagkistrodon acutus Venom-Induced Acute Kidney …Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabraMenthacarin attenuates experimental colitisImmunomodulatory effects of sCD83 via induction of TGF-β1 and IDO: an in vitro and rat orthotopic liver transplantation studyExperimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction by enhancing Th2 cytokineConvallatoxin Promotes M2 Macrophage Polarization to Attenuate Atherosclerosis Through PPAR¦Ã-Integrin ¦Áv¦Â5 Signaling PathwayAntiangiogenic Effect of Platelet P2Y12 Inhibitor in Ischemia-Induced Angiogenesis in Mice HindlimbAsiatic acid attenuates diabetic retinopathy through TLR4/MyD88/NF-¦ÊB p65 mediated modulation of microglia polarizationBeneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studiesMelatonin ameliorates anxiety-like behaviors induced by sleep deprivation in mice: role of oxidative stress, neuroinflammation, autophagy and apoptosisThe Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancementEstablishing novel roles of bifidocin LHA, antibacterial, antibiofilm and immunomodulator against Pseudomonas aeruginosa corneal infection modelInhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial VirusEthanolic extract of Pyrus pashia buch ham ex. D. 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ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CSIF,IL10A,TGIF,Cytokine Synthesis Inhibitory Factor
Available
Specificity	Mus musculus (Mouse)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interleukin 10 (IL10). No significant cross-reactivity or interference between Interleukin 10 (IL10) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 (IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10 (IL10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Infection immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.