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ELISA Kit for Interleukin 10 (IL10)

ELISA Kit for Interleukin 10 (IL10)

Item no.	SEA056Ra-5x96T
Manufacturer	Cloud-Clone
Amount	5x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Colorectal Cancer Testicular & Prostate Cancer
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 3.5pg/mL
Citations	New Method to Isolate and Culture Rat Kupffer CellsAttenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat modelBerberine alleviates preeclampsia possibly by regulating the expression of interleukin-2/interleukin-10 and Bcl-2/BaxEffect of remote ischemic preconditioning in the expression of IL-6 and IL-10 in a rat model of liver ischemia-reperfusion injuryMesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE5 and is crucial for liver transplant toleranceVSL# 3 probiotic treatment decreases bacterial translocation in rats with carbon tetrachloride‐induced cirrhosisSignificance of the changes occurring in the levels of interleukins, SOD and MDA in rat pulmonary tissue following exposure to different altitudes and exposure times Effects of prebiotic supplementation on the expression of proteins regulating iron absorption in anaemic growing ratsЦитокOxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathways白细胞介素10 上调表达对家兔慢性细菌性鼻窦炎黏膜创伤修复的影响Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes.Effect of Passion Fruit (Passiflora edulis f. flavicarpa deg.) Peel Flour on the Prognosis of Acute Pancreatitis after Overnutrition During LactationTransplantation of a bacterial consortium ameliorates trinitrobenzenesulfonic acid-induced colitisand intestinal dysbiosis in rats.Comparison of human adipose stromal vascular fraction and adipose-derived mesenchymal stem cells for the attenuation of acute renal ischemia/reperfusion injury.Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritisCardioprotective time-window of Penehyclidine hydrochloride postconditioning: A rat studyComparison of human adipose stromal vascular fraction and adipose-derived mesenchymal stem cells for the attenuation of acute renal ischemia/reperfusion injuryHigh-dose dexamethasone induced LPS-stimulated rat alveolar macrophages apoptosis.EGCG Maintains Th1/Th2 Balance and MitigatesUlcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-ݜ傠Signaling Pathway in RatsTherapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in ratsProtective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed ratsEGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-κB Signaling Pathway in RatsInfluence of Vitamin D Deficiency on Progression of Experimental Otitis Media in RatsTualang Honey Reduced Neuroinflammation and Caspase-3 Activity in Rat Brain after Kainic Acid-Induced Status EpilepticusS‑adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient …Gastroprotective effect of apricot kernel oil in ethanol-induced gastric mucosal injury in ratsInhibition of Rho kinase by GSK 269962 reverses both corticosterone-induced detrusor overactivity and depression-like behaviour in ratsChronic hyperammonemia induces peripheral inflammation that leads to cognitive impairment in rats: reversal by anti-tnfa treatmentAnti-inflammatory and cognitive effects of interferon-β1a (IFNβ1a) in a rat model of Alzheimer's diseaseERK activation by zeranol has neuroprotective effect in cerebral ischemia reperfusionTHE ROLE OF ANTI-INFLAMMATORY CYTOKINES IN MEMORY PROCESSING IN A HEALTHY BRAINThe wound healing potential of a pro-angiogenic peptide purified from Indian Russell's viper (Daboia russelii) venomNetwork pharmacology oriented study reveals inflammatory state-dependent dietary supplement hepatotoxicity responses in normal and diseased ratsPinealektomize Edilmiş Septik Sıçanlarda TNF-α, IL-6, IL-10 ve IL-1β'nin Kan Düzeyleri ile Karaciğer ve Böbrekte Gen Ekspresyonu Seviyelerindeki …Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in ratProtective effect of Vitamin D3 against lead induced hepatotoxicity, oxidative stress, immunosuppressive and calcium homeostasis disorders in ratCrocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosisAnti-inflammatory and antioxidant effects of muscarinic acetylcholine receptor (mAChR) activation in the rat hippocampusKappa opioid receptor agonists improve postoperative cognitive dysfunction in rats via the JAK2/STAT3 signaling pathwayQingkailing injection ameliorates cerebral ischemia-reperfusion injury and modulates the AMPK/NLRP3 Inflammasome Signalling pathwayNonsteroidal anti‐inflammatory drugs alleviate severity of post‐ERCP pancreatitis by inhibiting inflammation and reducing apoptosisHydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTOR/TFEB …Irisin Improves Autophagy of Aged Hepatocytes via Increasing Telomerase Activity in Liver InjuryExosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 …Management of Citrus sinensis peels for protection and treatment against gastric ulcer induced by ethanol in ratsChlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stressEffect of procyanidins on lipid metabolism and inflammation in rats exposed to alcohol and ironDownregulation of ROCK2 attenuates alcohol-induced inflammation and oxidative stress in astrocytesDifferent Activation of IL-10 in the Hippocampus and Prefrontal Cortex During Neurodegeneration Caused by Trimethyltin ChlorideDown-regulation of ROCK2 alleviates ethanol-induced cerebral nerve injury partly by the suppression of the NF-κB signaling pathwayA potential drug combination of omeprazole and patchouli alcohol significantly normalizes oxidative stress and inflammatory responses against gastric ulcer in ethanol …κ‑opioid receptor agonists may alleviate intestinal damage in cardiopulmonary bypass rats by inhibiting the NF‑κB/HIF‑1α pathwayPlacenta‑derived mesenchymal stem cells ameliorate lipopolysaccharide‑induced inflammation in RAW264. 7 cells and acute lung injury in ratsVitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular …Wine pomace product modulates oxidative stress and microbiota in obesity high-fat diet-fed ratsPhytochemical and Toxicological Effect of Ethanol Extract of Heliotropium Indicum on Liver of Male Albino RatsDietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet.Effects of minocycline on neuroinflammation, gut microbiome and hippocampal neurogenesis in rats with Gulf War IllnessMinocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal NeurogenesisMalted Barley Ameliorated Spinal Cord Injuries of Offspring of Hypercholesterolemic Mother RatsUmbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in ratsAnti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat ModelMinocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesisp-Coumaric acid ameliorates ionizing radiation-induced intestinal injury through modulation of oxidative stress and pyroptosisUltrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves?¡Dobera glabra (Forssk.) Poir.(Salvadoraceae) phenolic constituents of the aqueous leaves extract and evaluation of its anti-inflammatory, analgesic activitiesThe Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway RegulationComparative Study on the Pro-Inflammatory Activity of Turmeric (Curcuma longa) and flaxseed (Linumusitatissimum)Treadmill Running Changes Endothelial Lipase Expression: Insights from Gene and Protein Analysis in Various Striated Muscle Tissues and SerumAlterations in the gut microbiome with hemorrhagic transformation in experimental strokeThymol ameliorates 5‐fluorouracil‐induced intestinal mucositis: Evidence of down‐regulatory effect on TGF‐β/MAPK pathways through NF‐κBVitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in ratAdministration of a Probiotic Mixture Ameliorates Cisplatin-Induced Mucositis and Pica by Regulating 5-HT in RatsProtective Effects of Carbon Dots Derived from Armeniacae Semen Amarum Carbonisata Against Acute Lung Injury Induced by Lipopolysaccharides in RatsProtective antioxidative and anti-inflammatory actions of β-caryophyllene against sulfasalazine-induced nephrotoxicity in ratJiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axisSilymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathwayPhotobiomodulation associated with lipid nanoparticles and hyaluronic acid accelerate the healing of excisional woundsBio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine Phytochemical, Antioxidant, Anti-Inflammatory …Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in ratsDexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA SignalingPromising Therapeutic Efficacy of Trigonella-foenum graecum and Bone Marrow-Derived Mesenchymal Stem Cells on Skeletal Muscle Atrophy in Experimental …
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CSIF,IL10A,TGIF,Cytokine Synthesis Inhibitory Factor
Available
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interleukin 10 (IL10). No significant cross-reactivity or interference between Interleukin 10 (IL10) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 (IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10 (IL10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Infection immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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