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ELISA Kit for Interleukin 17 (IL17)

ELISA Kit for Interleukin 17 (IL17)

Item no.	SEA063Ra-48T
Manufacturer	Cloud-Clone
Amount	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neuroinflammation Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Testicular & Prostate Cancer
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 6.4pg/mL
Citations	Impaired Autoimmune T Helper 17 Cell Responses Following DNA Vaccination against Rat Experimental Autoimmune Encephalomyelitis Atorvastatin ameliorates experimental autoimmune neuritis by decreased Th1/Th17 cytokines and up-regulated T regulatory cells Improvement by Phytotherapeutic Agent of Detrusor Overactivity, Down-Regulation of Pharmacological Receptors and Urinary Cytokines in Rats with Cyclophosphamide Induced Cystitis Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced Cystitis Expression of Hemopexin in Acute Rejection of Rat Liver Allograft Identified by Serum Proteomic Analysis. Pemetrexed Ameliorates Experimental Arthritis in Rats Lapatinib Ameliorates Experimental Arthritis in Rats Protective effect and mechanism of probucol in the treatment of spinal cord injury Tanshinone IIA attenuates experimental autoimmune encephalomyelitis in rats Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Antiinflammatory and antioxidant effects of gemcitabine in collagen-induced arthritis model Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis Therapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in rats Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory … Negative regulation of glial Tim‐3 inhibits the secretion of inflammatory factors and modulates microglia to antiinflammatory phenotype after experimental intracerebral?… Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat Epigallocatechin 3‐gallate attenuates arthritis by regulating Nrf‐2, HO‐1 and cytokine levels in an experimental arthritis model Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular … Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet. Effects of minocycline on neuroinflammation, gut microbiome and hippocampal neurogenesis in rats with Gulf War Illness Minocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal Neurogenesis 丹参对非酒精性脂肪肝Th17细胞及相关细胞因子的影响* Minocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesis Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of ¦Á1, 3 ¡ Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	IL17A,CTLA8,IL-17A,Cytotoxic T-Lymphocyte-Associated Protein 8
Available
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17). No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 17 (IL17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 17 (IL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 17 (IL17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 17 (IL17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Infection immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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