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ELISA Kit for Interleukin 4 (IL4)

ELISA Kit for Interleukin 4 (IL4)

Item no.	SEA077Hu-5x96T
Manufacturer	Cloud-Clone
Amount	5x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 5.9pg/mL
Citations	Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesityThe immunomodulating effect of seminal plasma on T cellsThe effects of Spirulina platensis (Arthrospira platensis) and Saccharomyces cerevisiae on the distribution and cytokine production of CD4+ and CD8+ T …Evaluation of Cell-mediated Immune Response in PBMCs of Calves Vaccinated by Capri Pox Vaccines Using ELISA and Real-time RT-PCROptimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell SheetsSystemic and Tumor Th1 and Th2 Inflammatory Profile and Macrophages in Lung Cancer: Influence of Underlying Chronic Respiratory Disease.Epstein Barr Virus Infection Affects Function of Cytotoxic T Lymphocytes in Patients with Severe Aplastic Anemiaخصوصیات پاسخ‌های ایمنی القاء شده ناشی از واکسن زنده تخفیف حدت یافته آبله بزی سویه گرگان بر علیه بیماری لمپی اسکین در گاو‎Protective effects of polydatin in free and nanocapsulated form on changes caused by lipopolysaccharide in hippocampal organotypic culturesConstruction and Immunogenicity of Novel Chimeric Virus-Like Particles Bearing Antigens of Infectious Bronchitis Virus and Newcastle Disease VirusPreclinical study of safety and immunogenicity of combined rubella and human papillomavirus vaccines: Towards enhancing vaccination uptake rates in …Antibacterial immunomodulatory and antibiofilm triple effect of Salivaricin LHM against Pseudomonas aeruginosa urinary tract infection modelActive smoking, sleep quality and cerebrospinal fluid biomarkers of neuroinflammationQingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic miceTalaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patientsCytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control studyAssociations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive …The microRNA-1278/SHP-1/STAT3 pathway is involved in airway smooth muscle cell proliferation in a model of severe asthma both intracellularly and extracellularly
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	BSF1,BCGF1 ;B Cell Stimulatory Factor 1,Lymphocyte stimulatory factor 1,B Cell Growth Factor,Binetrakin,Pitrakinra
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interleukin 4 (IL4). No significant cross-reactivity or interference between Interleukin 4 (IL4) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 (IL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 4 (IL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 4 (IL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 4 (IL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Immune molecule;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.