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ELISA Kit for Interleukin 6 (IL6)

ELISA Kit for Interleukin 6 (IL6)

Item no.	SEA079Bo-5x96T
Manufacturer	Cloud-Clone
Amount	5x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Metabolism Diabetes Glucose Homeostasis By Cell Type Carcinoma Hematology Leukemia Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Testicular & Prostate Cancer Bone Cancer Uterine, Ovarian & Cervical Cancer
Type	Elisa-Kit
Specific against	Cow (Bos taurus), Cattle (Bovine)
Sensitivity	The minimum detectable dose of this kit is typically less than 3.0pg/mL
Citations	The Concentrations of Inflammatory Cytokines and Acute‐Phase Proteins in the Peripheral Blood and Uterine Washings in Cows with Pyometra Inflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum. Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes Inflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum Inflammatory cytokine and acute phase protein concentrations in the peripheral blood and uterine washings of cows with subclinical endometritis in the late postpartum period АНАЛИЗ ЦИТОКИНОВОГО СТАТУСА ПРИ ОРВИ КРУПНОГО РОГАТОГО СКОТА Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells Acute phase response in the primiparous dairy cows after repeated percutaneous liver biopsyduring the transition period. Milk and serum IL-4, IL-6, IL-10, and amyloid A concentrations in cows with subclinical mastitis caused by coagulase-negative staphylococci Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows A study of altered cytokine rhythms associated with successful implantation in cows Relationship between body condition score and plasma adipokines in early-lactating Holstein dairy cows Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos … MAVS induces a host cell defense to inhibit CSFV infection Milk fever in dairy cows is preceded by activation of innate immunity and alterations in carbohydrate metabolism prior to disease occurrence Effect of reproductive disorders on productivity and reproductive efficiency of dromedary she-camels in relation to cytokine concentration Rifaximin anti‐inflammatory activity on bovine endometrium primary cell cultures: a preliminary study Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows Levels of selected cytokines and acute–phase proteins in the serum of dairy cows with cystic ovarian disease and those in follicular and luteal phases of normal … Early cytokine response after vaccination with Coxiella burnetii phase I in an infected herd of dairy cattle Effective Treatment of Bovine Mastitis with Intramammary Infusion of Angelica dahurica and Rheum officinale Extracts Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows The association of circulating prepartum metabolites, minerals, cytokines and hormones with postpartum health status in dairy cattle Mitochondrial membrane protein mitofusin 2 as a potential therapeutic target for treating free fatty acid–induced hepatic inflammation in dairy cows during early … A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy … Integrated effects of season and parturition-associated stress on the inflammatory response and metabolic status in Sahiwal (Bos Indicus) cows Comparative evaluation of cytokine and acute-phase protein concentrations in sera of dairy cows with subclinical and clinical ketosis as a different view of the ¡
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MGI2-A,MGI2A,HGF,BSF2,HSF,IFNB2,B-Cell Stimulatory Factor-2,Hybridoma/Plasmacytoma Growth Factor,Hepatocyte Stimulating Factor,Cytotoxic T-Cell Differentiation Factor
Available
Specificity	Bos taurus; Bovine (Cattle)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6). No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Tumor immunity; Infection immunity; Cardiovascular biology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.