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ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)

ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)

Item no.	SEA087Hu-48T
Manufacturer	Cloud-Clone
Amount	48 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Infectious Diseases Viral Infections Coronavirus (COVID-19) ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Skin Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 6.4pg/mL
Citations	Serum levels of selected chemokines in systemic lupus erythematosus patients MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cells MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1 НАРУШЕНИЕ БАЛАНСА ХРОМА И ВАНАДИЯ В ЖИРОВОЙ ТКАНИ КАК ВОЗМОЖНЫЙ МЕХАНИЗМ ОЖИРЕНИЕ-АССОЦИИРОВАННОЙ ИНСУЛИНОРЕЗИСТЕНТНОСТИ Anti‐inflammatory properties of tianeptine on lipopolysaccharide‐induced changes in microglial cells involve toll‐like receptor‐related pathways The serum concentrations of leptin and MCP-1 independently predict low back pain duration. oxLDL antibody inhibits MCP‐1 release in monocytes/macrophages by regulating Ca2+/K+ channel flow Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity Increased Serum MCP-1 Levels in Systemic Vasculitis Patients with Renal Involvement Long non-coding RNA mitigates atherosclerosis by regulating the actin-binding protein NEXN A sensitive sandwich-type immunosensor for the detection of MCP-1 based on a rGO-TEPA-Thi-Au nanocomposite and novel RuPdPt trimetallic nanoalloy particles Effect of stress hyperglycaemia on monocyte chemoattractant protein‑1 levels and the short‑term prognosis of patients with acute ST‑segment elevation myocardial … LncRNA HOXA11‐AS regulates calcium oxalate crystal–induced renal inflammation via miR‐124‐3p/MCP‐1 Modulating endothelial adhesion and migration impacts stem cell therapies efficacy Effects of regular exercise on inflammatory biomarkers and lipid parameters in soccer players CCL2 (CC Motif Chemokine Ligand 2) Biomarker Responses in Central Versus Peripheral Compartments After Focal Cerebral Ischemia
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CCL2,GDCF2,HC11,HSMCR30,MCAF,MCP1,SCYA2,SMC-CF,Chemokine C-C-Motif Ligand 2,Monocyte Chemotactic And Activating Factor,Monocyte Secretory Protein JE
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 1 (MCP1). No significant cross-reactivity or interference between Monocyte Chemotactic Protein 1 (MCP1) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monocyte Chemotactic Protein 1 (MCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Monocyte Chemotactic Protein 1 (MCP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Tumor immunity; Infection immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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