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ELISA Kit for Matrix Metalloproteinase 2 (MMP2)

ELISA Kit for Matrix Metalloproteinase 2 (MMP2)

Item no.	SEA100Hu-96T
Manufacturer	Cloud-Clone
Amount	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Food Safety Proteins Neuroscience Neuroinflammation Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Aging & Neurological Disorders By Cell Type Blastoma Cell Biology ELISA Cell Stainings ECM Cardiovascular Research Atherosclerosis ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Colorectal Cancer Breast Cancer Testicular & Prostate Cancer Gallbladder Cancer Gastric Cancer Pancreatic Cancer Hepatic Cancer Bone Cancer Uterine, Ovarian & Cervical Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 12.3pg/mL
Citations	Bone morphogenetic protein-2: a potential regulator in scleral remodelingThe Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary MenAcidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cellsDownregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesRNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cellsCan effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients?Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative courseCadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritisIncreased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasionA Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary MenMicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathwaysUltrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V3+year=2015volume=2issue=5spage=266epage=271aulast=Honnegowda">A comparative study to evaluate the effect of limited access dressing on diabetic ulcers1, 25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathwayMicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BAB1From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugatesMetalloproteinases and Their Inhibitors in Patients with Inguinal Hernia.Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases.Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival FibroblastsLiver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activityMicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer CellsFrom varices to venous ulceration: the story of chronic venous disease described by metalloproteinasesEpigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosisCellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathwaySyntenin-targeted peptide blocker inhibits progression of cancer cellsMatrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia …sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasionCD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular RemodelingEffect of a synthetic inhibitor of urokinase plasminogen activator on the migration and invasion of human cervical cancer cells in vitromiRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cellsHMGB1-RAGE signaling pathway in pPROMMMP-2 and TIMP-2 in patients with heart failure and chronic kidney diseaseMiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1Correlation of Baseline Plasma and Inguinal Connective Tissue Metalloproteinases and Their Inhibitors With Late High-Pressure Endoleak After Endovascular …MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16Stiffness of the Aligned Fibers Affects Structural and Functional Integrity of the Oriented Endothelial CellsExtracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertensionA novel role of VEPH1 in regulating AoSMC phenotypic switchingAlteration on MMP-2 Levels in Women Diabetes Patients in BasrahInvestigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheepGATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expressionMatrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe PreeclampsiaCalcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric CancerPlasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot StudyDownregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CLG4,CLG4A,MMP-II,MONA,TBE-1,PEX,Gelatinase A,72kDa Gelatinase,72kDa Type IV Collagenase
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 2 (MMP2). No significant cross-reactivity or interference between Matrix Metalloproteinase 2 (MMP2) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 2 (MMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 2 (MMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 2 (MMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 2 (MMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Enzyme & Kinase; Tumor immunity; Infection immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.