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ELISA Kit for Paraoxonase 1 (PON1)

ELISA Kit for Paraoxonase 1 (PON1)

Item no.	SEA243Hu-5x96T
Manufacturer	Cloud-Clone
Amount	5x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Neural Development ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Hepatic Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 1.21ng/mL
Citations	Fatty Liver Is Associated with Recurrent Bacterial Infections Independent of Metabolic SyndromePersistent elevation of paraoxonase-1 specific enzyme activity after weight reduction in obese non-diabetic men with metabolic syndromeCombined Serum Proteomic and Metabonomic Profiling After Laparoscopic Sleeve Gastrectomy in Children and AdolescentsHigh density lipoprotein structural changes and drug response in lipidomic profiles following the long-term fenofibrate therapy in the FIELD substudyMacrophage cholesterol efflux to plasma and HDL in subjects with low and high homocysteine levels: a FIELD substudyBeneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteomeExtra-virgin olive oil consumption reduces the age-related decrease in HDL and paraoxonase 1 anti-inflammatory activitiesParaoxonase 1: A better atherosclerotic risk predictor than HDL in type 2 diabetes mellitusParaoxonase responses to exercise and niacin therapy in men with metabolic syndromeA single session of aerobic exercise influences paraoxonase 1 activity and concentrationPlasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with CirrhosisAssociation of lipid profile with serum PON1 concentration in patients with chronic kidney diseaseThe Role of PON-1, GR, IL-18, and oxLDL inDepression With and Without Posttraumatic Stress DisorderAssociation of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertensionParaoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot Study in North Indian populationA Case-control Study: The Association of Serum Paraoxonase 1 Activity and Concentration with the Development of Type 2 Diabetes MellitusApplication of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosisThe role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder.Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levelsSerum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney DiseaseDifferentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktailsComparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancyPhenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intakeEstimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteinsThe HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation …PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patientsThe adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunctionMulti-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humansVitamin D status and systemic redox biomarkers in adults with obesityRepetitions of Strenuous Exercise Consistently Increase Paraoxonase 1 Concentration and Activity in Plasma of Average-Trained Men
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ESA,PON,Esterase A,Serum paraoxonase/arylesterase 1,Aromatic esterase 1,A-esterase 1,Serum aryldialkylphosphatase 1
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Paraoxonase 1 (PON1). No significant cross-reactivity or interference between Paraoxonase 1 (PON1) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paraoxonase 1 (PON1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paraoxonase 1 (PON1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paraoxonase 1 (PON1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paraoxonase 1 (PON1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paraoxonase 1 (PON1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paraoxonase 1 (PON1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Metabolic pathway; Endocrinology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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