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ELISA Kit for Matrix Metalloproteinase 9 (MMP9)

ELISA Kit for Matrix Metalloproteinase 9 (MMP9)

Item no.	SEA553Mu-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Oncology Neuroscience Neuroinflammation Metabolism Glucose Homeostasis By Cell Type Cell Biology ELISA Cell Stainings ECM Cardiovascular Research Atherosclerosis ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Bone Cancer
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.059ng/mL
Citations	Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells Increased Plasma Matrix Metalloproteinase-9 Levels Contribute to Intracerebral Hemorrhage during Thrombolysis after Concomitant Stroke and Influenza Infection Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells. The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female. Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF. Targeting peptidylarginine deiminase reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitis TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK … Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	GELB,Gelatinase B,CLG4B,CLG4-B,92 KDa Gelatinase,92kDa Type IV Collagenase
Available
Specificity	Mus musculus (Mouse)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 9 (MMP9). No significant cross-reactivity or interference between Matrix Metalloproteinase 9 (MMP9) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 9 (MMP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 9 (MMP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 9 (MMP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 9 (MMP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Enzyme & Kinase; Tumor immunity; Infection immunity; Cardiovascular biology; Hepatology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.