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ELISA Kit for Caspase 3 (CASP3)

ELISA Kit for Caspase 3 (CASP3)

Item no.	SEA626Mu-10x96T
Manufacturer	Cloud-Clone
Amount	10x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Neuroscience Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Aging & Neurological Disorders Protein Aggregation, Clearance & Misfolding Cell Biology Cell Status Apoptosis ELISA Cardiovascular Research Ischemia & Reperfusion Injury ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.229ng/mL
Citations	Role of mitochondria in ciprofloxacin-induced apoptosis in murine sperm cells.Vitamin D ameliorates hepatic ischemic/reperfusion injury in ratsInhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritisInhibition of small HA fragment activity and stimulation of A2AThe heat shock protein 90 inhibitor, 17-AAG, attenuates thioacetamide induced liver fibrosis in miceAn integrated proteomics approach shows synaptic plasticity changes in an APP/PS1 Alzheimers mouse modelAnti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in miceChronic low-dose-rate ionising radiation affects the hippocampal phosphoproteome in the ApoE−/− alzheimer mouse modelProtective effects of apigenin and myricetin against cisplatin-induced nephrotoxicity in mice.Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice.Tectorigenin attenuates the MPP+ induced SH‑SY5Y cell damage, indicating a potential beneficial role in Parkinson's disease by oxidative stress inhibitionPitavastatin inhibits proinflammatory cytokines and provides improvement in terms of its motor function in secondary injuries of the spinal cord mouse modelsPotential immunomodulatory and antioxidant effects of walnut vegetable oil against lead‐mediated hepatic damage and their interaction with lipase …Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino miceMebendazole augments sensitivity to sorafenib by targeting MAPK and BCL-2 signalling in n-nitrosodiethylamine-induced murine hepatocellular carcinomaChromon-3-aldehyde derivatives restore mitochondrial function in rat cerebral ischemia
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CPP32,CPP32B,SCA1,Apoptain,Yama,Apoptosis-Related Cysteine Peptidase,Cysteinyl Aspartate Specific Proteinases 3,SREBP cleavage activity 1
Available
Specificity	Mus musculus (Mouse)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Caspase 3 (CASP3). No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 3 (CASP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 3 (CASP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 3 (CASP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 3 (CASP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Signal transduction; Enzyme & Kinase; Apoptosis; Tumor immunity; Infection immunity;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.