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ELISA Kit for Resistin (RETN)

ELISA Kit for Resistin (RETN)

Item no.	SEA847Ra-96T
Manufacturer	Cloud-Clone
Amount	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Food Safety Hormones Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.64ng/mL
Citations	Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle Adipose tissue regulates hepatic cholesterol metabolism via adiponectin Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Tinospora crispa Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. Biological effects of adipocytes in sulfur mustard induced toxicity Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats Mango leaf tea promotes hepatoprotective effects in obese rats Bacupari (Garcinia brasiliensis) extract modulates intestinal microbiota and reduces oxidative stress and inflammation in obese rats Extruded sorghum (Sorghum bicolor L.) improves gut microbiota, reduces inflammation, and oxidative stress in obese rats fed a high-fat diet Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncoupler
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ADSF,FIZZ3,RETN1,RSTN,XCP1,Adipose tissue-specific secretory factor,C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein,Cysteine-rich secreted FIZZ3
Available
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Resistin (RETN). No significant cross-reactivity or interference between Resistin (RETN) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin (RETN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin (RETN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin (RETN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin (RETN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin (RETN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Resistin (RETN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Metabolic pathway; Endocrinology; Cardiovascular biology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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