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ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)

ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)

Item no.	SEA855Ra-96T
Manufacturer	Cloud-Clone
Amount	96 Tests
Quantity options	10x96 Tests 24 Tests 48 Tests 5x96 Tests 96 Tests
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lymphatic System Cancer
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 5.7pg/mL
Citations	The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells Effect of Nicotine on RANKL and OPG and Bone Mineral Density Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats A novel model of bisphosphonate-related osteonecrosis of the jaw in rats Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity. Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experiment The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats Improved RANKL expression and osteoclastogenesis induction of CD27+ CD38− memory B cells: A link between B cells and alveolar bone damage in periodontitis Alendronate Effect in RANKL Concentration at Ovariectomized Sprague Dawley Rat. Development and characterization of supramolecular calcitonin assembly and assessment of its interactions with the bone remodelling process Evaluation of therapeutic effects of teriparatide in a rat model of zoledronic acid-induced bisphosphonate-related osteonecrosis IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathway Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD254,TNFSF11,ODF,OPGL,TRANCE,HRANKL2,SOdf,Tumor Necrosis Factor(ligand)superfamily Member 11,TNF-related activation-induced cytokine
Available
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). No significant cross-reactivity or interference between Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Signal transduction; Metabolic pathway; Apoptosis; Tumor immunity; Bone metabolism;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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