ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO)

0.312-20ng/mL

The minimum detectable dose of this kit is typically less than 0.118ng/mL

3h

This assay has high sensitivity and excellent specificity for detection of Indoleamine-2, 3-Dioxygenase (IDO).
No significant cross-reactivity or interference between Indoleamine-2, 3-Dioxygenase (IDO) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Indoleamine-2, 3-Dioxygenase (IDO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Indoleamine-2, 3-Dioxygenase (IDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Indoleamine-2, 3-Dioxygenase (IDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Indoleamine-2, 3-Dioxygenase (IDO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects; Potential immunosuppressive function of plasma indoleamine 2, 3-dioxygenase in patients with aGVHD after allo-HSCT; Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell; CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms; Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2, 3-dioxygenase production in hepatocellular carcinoma; Indoleamine-2, 3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans; The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered; Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes; Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties; Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase; Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells;