ELISA Kit for Netrin 1 (Ntn1)

31.2-2, 000pg/mL

The minimum detectable dose of this kit is typically less than 11.3pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Netrin 1 (Ntn1).
No significant cross-reactivity or interference between Netrin 1 (Ntn1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Netrin 1 (Ntn1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Netrin 1 (Ntn1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Netrin 1 (Ntn1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Netrin 1 (Ntn1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Netrin-1: A Novel Universal Biomarker of Human Kidney Injury; Plasma netrin-1 is a diagnostic biomarker of human cancers; Cord blood netrin-1 and -4 concentrations in term pregnancies with normal, restricted and increased fetal growth; Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity; Cord blood netrin-1 and-4 concentrations in term pregnancies with normal, restricted and increased fetal growth; Urinary netrin-1 and KIM-1 as early biomarkers for septic acute kidney injury; Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC); The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium.; Relationship of serum netrin-1 levels with breast masses; Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus;