ELISA Kit for Hepcidin (Hepc)

62.5-4, 000pg/mL

The minimum detectable dose of this kit is typically less than 25.2pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc).
No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepcidin (Hepc). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepcidin (Hepc). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepcidin (Hepc), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepcidin (Hepc) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice; In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution.; Effects of iron supplementation in mice with hypoferremia induced by obesity; S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor,  Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway.; Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expression; inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages;