« Back
Home /
ELISA Kit for Interleukin 35 (IL35)

ELISA Kit for Interleukin 35 (IL35)

Item no.	SEC008Hu-24T
Manufacturer	Cloud-Clone
Amount	24 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	ELISA & Immunoassays ELISA Kits
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	The minimum detectable dose of this kit is typically less than 5.5pg/mL
Citations	Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature Increased plasma concentrations of interleukin 35 in patients with coronary artery disease The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析 Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferation IL‑35 may maintain homeostasis of the immune microenvironment in periodontitis Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritis Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. Evaluation of serum interleukin- 35 level in children with persistent asthma. Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation Clinical significance of IL-35 expression inthe progression of clear cell renal cell carcinoma Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy. AB0734 Serum il-35 levels in systemic sclerosis and relationship with clinical features Interleukin-35 as a New Biomarker of Renal Involvement in Lupus Nephritis Patients The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility Serum Interleukin 35 Levels in Systemic Sclerosis and Relationship With Clinical Features. Interleukin‑35 is associated with the tumorigenesis and progression of prostate cancer CD4+ FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunity Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain Association of IL-35 expression and gene polymorphisms in rheumatoid arthritis Decreased interleukin-35 levels and CD4+ EBI3+ T cells in patients with type 1 diabetes and the effects of the antibody against CD20 (rituximab) Anti-inflammatory roles of interleukin-35 in the pathogenesis of Japanese cedar pollinosis Association of three single nucleotide polymorphisms of interleukin-35 for detection and prediction of women with breast cancer in Erbil city Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Specificity	Homo sapiens (Human)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interleukin 35 (IL35). No significant cross-reactivity or interference between Interleukin 35 (IL35) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 35 (IL35) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 35 (IL35) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to IL12A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated monoclonal antibody specific to IL27B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Tumor immunity; Infection immunity

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.