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ELISA Kit for Catalase (CAT)

ELISA Kit for Catalase (CAT)

Item no.	SEC418Ra-5x96T
Manufacturer	Cloud-Clone
Amount	5x96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite Cell Biology Cell Status ELISA Oxidative Stress ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.064ng/mL
Citations	Phloroglucinol protects gastric mucosa against ethanol-induced injury through regulating myeloperoxidase and catalase activities Antioxidant profile of salivary glands in high fat diet-induced insulin resistance rats Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar rats Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formation Selected elements of extracellular matrix of the skin in diabetes and insulin resistance European Journal of Biomedical AND Pharmaceutical sciences Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring Toxic Trace Metals and Pathological Changes in Organs of Rats Fed with Extract of Polluted Grasses Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡ Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡ Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine Phytochemical, Antioxidant, Anti-Inflammatory …
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Specificity	Rattus norvegicus (Rat)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Catalase (CAT). No significant cross-reactivity or interference between Catalase (CAT) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catalase (CAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catalase (CAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catalase (CAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catalase (CAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catalase (CAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catalase (CAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Enzyme & Kinase; Metabolic pathway; Hepatology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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