ELISA Kit for Klotho (KL)

15.6-1, 000pg/mL

The minimum detectable dose of this kit is typically less than 6.0pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Klotho (KL).
No significant cross-reactivity or interference between Klotho (KL) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Klotho (KL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Klotho (KL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Klotho (KL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Klotho (KL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature; Les biomarqueurs des calcifications vasculaires; Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho; Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3; Soluble Klotho protein as a novel serum biomarker in patients with acromegaly; Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management?; Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications; The role of klotho in chronic kidney disease;