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ELISA Kit for Casein Beta (CSN2)

ELISA Kit for Casein Beta (CSN2)

Item no.	SEJ332Bo-96T
Manufacturer	Cloud-Clone
Amount	96 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	Food Safety Allergenes Proteins Cell Biology Cell Status ELISA Senescence ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Cow (Bos taurus), Cattle (Bovine)
Sensitivity	The minimum detectable dose of this kit is typically less than 3.5ng/mL
Citations	A modified piggybac transposon system mediated by exogenous mRNA to perform gene delivery in bovine mammary epithelial cells Up-reCavia (Guinea pig )lation of milk secretion with modified microclimate through manipulating plasminogen-plasmin systemin Murrah buffaloes during hot dry season. Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season. Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells Inhibition of arginase via jugular infusion of Nω-hydroxy-nor-l-arginine inhibits casein synthesis in lactating dairy cows The influence of a modified micro-environment on stress and milk production through the plasminogen-plasmin system in Murrah buffaloes during the hot-humid … Arginine Supply Impacts the Expression of Candidate microRNA Controlling Milk Casein Yield in Bovine Mammary Tissue Rationally designed bioactive milk-derived protein scaffolds enhanced new bone formation
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CASb
Available
Specificity	Bos taurus; Bovine (Cattle)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	milk
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Casein Beta (CSN2). No significant cross-reactivity or interference between Casein Beta (CSN2) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Casein Beta (CSN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Casein Beta (CSN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Beta (CSN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Beta (CSN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Beta (CSN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Casein Beta (CSN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Signal transduction;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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