Mouse IL-4 ELISA Kit

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-4. The Mouse IL-4 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 4 (IL-4) is an approximately 18-20 kDa monomeric cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Mouse IL-4 is synthesized as a 140 amino acid (aa) precursor with a 20 aa signal sequence and a 120 aa mature chain and mature mouse IL-4 shares 43%, and 63% aa sequence identity with human and rat IL-4. IL-4 is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to IL-13. IL-4 also has been shown to drive mitogenesis, dedifferentiation, and metastasis in rhabdomyosarcoma. IL-4, along with other Th2 cytokines, is involved in the airway inflammation observed in the lungs of patients with allergic asthma.

Sandwich ELISA

serum, plasma, cell culture supernates

4C (unopened)
standard stored at -20C, others stored at 4C (opened)

0.34 pg/ml

93 % - 106 %

n.d. - 16.4 pg/ml

6.8 % - 8.3 %