Mouse IL-12/23(P40) ELISA Kit

ELISA Kit

TNF

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Interleukin-12 subunit beta

This assay recognizes both natural and recombinant human TNF-a. The factors listed below were prepared at 70ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: TNF-b, GM-CSF, s TNF RI, s TNF RII, TNF-b,
Recombinant mouse: TNF-a, TNF-a（truncated）, s TNF RI,
Recombinant porcine: TNF-a,

Components
2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)1 vial Substrate Solution 1 vial Stop Solution2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody

The coefficient of variation of both intra-assay and inter-assay were less than 10%.