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MCM5 ELISA Kit (Human)

MCM5 ELISA Kit (Human)

Item no.	OKCD00440
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Neuroscience Neural Lineage Markers ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.061 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CDC46;CDC46 homolog;DNA replication licensing factor MCM5;MCM5 minichromosome maintenance deficient 5,cell division cycle 46;MGORS8;minichromosome maintenance deficient 5 (cell division cycle 46);P1-CDC46.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	MCM5
Gene Fullname	minichromosome maintenance complex component 5
Protein size	734
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.
Nucleotide accession_num	NM_006739.3
Protein accession_num	NP_006730
Protein name	DNA replication licensing factor MCM5
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Minichromosome Maintenance Deficient 5 (MCM5).No significant cross-reactivity or interference between Minichromosome Maintenance Deficient 5 (MCM5) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich Immunoassay
Kit Components	Component: Amount Anti-MCM5 Microplate: 96 Wells (12 x 8 Well Strips) MCM5 Lyophilized Standard: 2 x 10 ng 100X Biotinylated MCM5 Detector Antibody: 120 uL Avidin/HRP Conjugate: 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL TMB Substrate: 1 x 9 mL Stop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Minichromosome Maintenance Deficient 5 (MCM5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Minichromosome Maintenance Deficient 5 (MCM5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Minichromosome Maintenance Deficient 5 (MCM5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Minichromosome Maintenance Deficient 5 (MCM5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Minichromosome Maintenance Deficient 5 (MCM5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Minichromosome Maintenance Deficient 5 (MCM5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 1/8/2026

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