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LAIR1 ELISA Kit (Human)

LAIR1 ELISA Kit (Human)

Item no.	OKCD07047
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Aging & Neurological Disorders By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Melanoma Sarcoma Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.129ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD305;immunoglobulin heavy chain variable region;LAIR-1;leukocyte-associated Ig-like receptor 1;leukocyte-associated immunoglobulin-like receptor 1.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	LAIR1
Gene Fullname	leukocyte associated immunoglobulin like receptor 1
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
Nucleotide accession_num	NM_001289023.2
Protein accession_num	NP_001275952.2
Protein name	Leukocyte-associated immunoglobulin-like receptor 1
Kit Components	Component: Amount LAIR1 Microplate: 96 Wells (12 x 8 Well strips)LAIR1 Lyophilized Standard: 2100X LAIR1 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma and other biological fluids.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD07047.pdf

OKCD07047-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/25/2025

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