ELISA Kit for Angiotensinogen (AGT)

3.12-200ng/mL

The minimum detectable dose of this kit is typically less than 1.31ng/mL

3h

This assay has high sensitivity and excellent specificity for detection of Angiotensinogen (AGT).
No significant cross-reactivity or interference between Angiotensinogen (AGT) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensinogen (AGT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensinogen (AGT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensinogen (AGT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensinogen (AGT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients?; The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients; Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension; Urinary angiotensinogen as a novel marker of obstructive nephropathy in children; Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia; MP707CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY; Relationship between blood pressure var; Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis; Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa; Relationship between blood pressure variability and renal activity of the renin-angiotensin system.; Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis; Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity; Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia;