Comparison

ELISA Kit for Alpha-1-Antichymotrypsin (a1ACT)

Manufacturer Cloud-Clone
Category
Type Elisa-Kit
Specific against Rat
Amount 5x96T
Item no. SEB015Ra-5x96T
eClass 6.1 32160605
eClass 9.0 32160605
Available
Alias A1-ACT; SERPINA3; AACT; ACT; GIG24; GIG25; Serpin Peptidase Inhibitor Clade A Member 3(Alpha-1 Antiproteinase; Antitrypsin); Cell growth-inhibiting gene 24/25 protein
Quantity options
Detection range
1.56-100ng/mL
Organism species
Rattus norvegicus (Rat)
Sensitivity
The minimum detectable dose of this kit is typically less than 0.74ng/mL
Sample type
Serum, plasma and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity

This assay has high sensitivity and excellent specificity for detection of Alpha-1-Antichymotrypsin (a1ACT).
No significant cross-reactivity or interference between Alpha-1-Antichymotrypsin (a1ACT) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Antichymotrypsin (a1ACT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Antichymotrypsin (a1ACT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antichymotrypsin (a1ACT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antichymotrypsin (a1ACT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antichymotrypsin (a1ACT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antichymotrypsin (a1ACT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Enzyme & Kinase; Infection immunity; Neuro science; Pulmonology; Hepatology;

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5x96T
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close