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BMP-2, Human

BMP-2, Human

Item no.	Z02913-50
Manufacturer	GenScript
Amount	50 ug
Quantity options	1 mg 10 ug 100 ug 50 ug
Category	Neuroscience Metabolism Diabetes Molecular Targets for Neuroscience Aging & Neurological Disorders Neural Signaling Infectious Diseases Viral Infections Coronavirus (COVID-19) Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Cell Status Cell Metabolism Cardiovascular Research Atherosclerosis Oxidative Stress
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	> 95% as analyzed by non-reducing SDS-PAGE.
Sequence	Gln283-Arg396 (accession number:P12643). Expressed with an N-terminal Met.
Citations	Sampath, T.K. et al. Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily. J Biol Chem. 1990 Aug 5,265(22):13198-205. Heng S. et al. Posttranslational activation of bone morphogenetic protein 2 is mediated by proprotein convertase 6 during decidualization for pregnancy establishment. Endocrinology. 2010 Aug,151(8):3909-17. Epub 2010 Jun 16. Celeste, A. J. et al. Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. Proc Natl Acad Sci U S A. 1990 Dec,87(24):9843-7.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02913_50-Bone_Morphogenetic_Protein_2_BMP_2_Human,Human Bone Morphogenetic Protein-2 (BMP-2), is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2), is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys360. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction. Recombinant human Bone Morphogenetic Protein-2 (rhBMP-2), produced in, E. coli, is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 115 amino acids. A fully biologically active molecule, rhBMP-2 has a molecular mass of 26 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> 26 kDa, observed by non-reducing SDS-PAGE</td></tr><tr><th>Purity</th><td colspan="7"> > 95% as analyzed by non-reducing SDS-PAGE.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> < 1 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> ED50 < 1 ug/ml, measured by alkaline phosphatase induction assay using C2C12 cells.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized, recombinant human Bone Morphogenetic Protein-2 (BMP-2), remains stable at room temperature for two weeks. Upon reconstitution, rhBMP-2 should be stable up to 1 month at 4C and up to 3 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against 50 mM acetic acid.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in 20 mM AcOH/5 mM HCl. The solubility should be at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> Gln283-Arg396 (accession number:P12643). Expressed with an N-terminal Met.</td></tr><tr><th>Reference</th><td colspan="7">Sampath, T.K. et al. Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily. J Biol Chem. 1990 Aug 5,265(22):13198-205. Heng S. et al. Posttranslational activation of bone morphogenetic protein 2 is mediated by proprotein convertase 6 during decidualization for pregnancy establishment. Endocrinology. 2010 Aug,151(8):3909-17. Epub 2010 Jun 16. Celeste, A. J. et al. Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. Proc Natl Acad Sci U S A. 1990 Dec,87(24):9843-7.</td></tr>
Similar products	Bone
Shipping Condition	Cool pack
Available
Specificity	ED50 < 1 ug/ml, measured by alkaline phosphatase induction assay using C2C12 cells.
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	26 kDa, observed by non-reducing SDS-PAGE
Product Line	Cytokines
Manufacturer - Specificity	ED50 < 1 ug/ml, measured by alkaline phosphatase induction assay using C2C12 cells.
Description	Human Bone Morphogenetic Protein-2 (BMP-2), is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2), is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys360. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction. Recombinant human Bone Morphogenetic Protein-2 (rhBMP-2), produced in, E. coli, is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 115 amino acids. A fully biologically active molecule, rhBMP-2 has a molecular mass of 26 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Reconstitution	Reconstituted in 20 mM AcOH/5 mM HCl. The solubility should be at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against 50 mM acetic acid.
Endotoxin Level	< 1 EU/ug, determined by LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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