Interleukin-10 (IL-10), Mouse

Interleukin-10 (IL-10), is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 (IL-10) in mice is Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce Interleukin-10 (IL-10). In human Interleukin-10 (IL-10) is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by mococytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cells lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secerete large quantities of Interleukin-10 (IL-10) into the conditioned medium. The synthesis of Interleukin-10 (IL-10) by monocytes is inhibited by IL-4 and IL-10. Recombinant mouse Interleukin-10 (IL-10), produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18, 885 Da.

The protein was lyophilized after extensive dialysis against 20mM PB, pH7.3, 300mM NaCl buffer.

Cytokine, Chemokines & Growth Factors

Lyophilized, Mouse Interleukin-10 (IL-10), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution Mouse Interleukin-10 (IL-10) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.