CD18

Manufacturer GENWAY
Category
Type Antibody
Specific against other
Clone YTS213. 1
Applications FC, IP, IHC
Amount 0.025 mg
ArtNr 20-783-310012
Available
Genway ID:
GWB-E1CD97
Specificity:
CD18NCBI
Gene ID:
16414
Specificity:
CD18
Clone:
YTS213. 1
Immunogen:
Mouse spleen cells
Fusion Partner:
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1. 2. 3 myeloma cell line.
Specificity Note:
Mouse CD18 (LFA-1 family b chain) is expressed on most leucocytes YTS 213. 1 does not block LFA-1 mediated T-cell activation in vitro. The antibody precipitates an antigen of 95-180kD.
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium AzideSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - 1/25
Function:
Integrin alpha-L/beta-2 is a receptor for ICAM1 ICAM2 ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Subunit:
Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L alpha-M alpha-X or alpha-D. Interacts with COPS5 and RANBP9.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Ptm:
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Disease:
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type I (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Similarity:
Belongs to the integrin beta chain family.
Similarity:
Contains 1 VWFA domain. 1. Madrid. S. et al. (1983) Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions. LFA-1 and Mac-1.
Amount: 0.025 mg
Available: In stock
Listprice: €152.38
Discount: -10.0%
Price: €137.14
You save: €15.24
lieferbar

Get an offer

Technische Fragen zu diesem Produkt?


Salesmanager: Arne Pelz

Email: sales@hoelzel.de

Close