CD62L


Manufacturer	GENWAY
Category	Oncology Neuroscience Neural Signaling Neural Adhesion Molecules Antibodies Cell Biology Cell Movement Cell Adhesion Flow Cytometry Immunohistochemistry Immunoprecipitation Primary Antibodies By Organ Heart & Blood Cancer
Type	Antibody
Specific against	other
Clone	FMC46
Applications	FC, IP, IHC
Amount	0.025 mg
Item no.	20-783-310016
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-C8EAEE
Specificity:	CD62LNCBI
Gene ID:	6402
Specificity:	CD62L
Clone:	FMC46
Immunogen:	PHA stimulated lymphoblasts.
Fusion Partner:	Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Histology: Human Tonsil
Preparation:	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution:	Phosphate buffered saline pH7. 4
Preservative Stabilisers:		0. 09%Sodium AzideSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:	Flow Cytometry - 1/50 - 1/100
Function:	Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Subunit:	Interaction with PSGL1/SELPLG is required for promoting recruitment and rolling of leukocytes. This interaction is dependent on the sialyl Lewis X glycan and tyrosine sulfation modifications of PSGL1. Sulfation on Y-51 of PSGL1 is important for L-selectin binding.
Subcellular Location:	Membrane; Single-pass type I membrane protein.
Tissue Specificity:	Expressed in B cell lines and T lymphocytes.
Similarity:	Belongs to the selectin/LECAM family.
Similarity:	Contains 1 C-type lectin domain.
Similarity:	Contains 1 EGF-like domain.
Similarity:	Contains 2 Sushi (CCP/SCR) domains. 1. Pilarski. L. M. et al. (1991) FMC46. a cell protrusion-associated leucocyte adhesion molecule-1 epitope on human lymphocytes and thymocytes. 2. Zola. H. et al. (1991) The expression of sub-population markers on B cells: A re-evaluation using high sensitivity fluorescence flow cytometry. 3. Sopp. P. and Howard. C. C. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte diferentiation antigens with bovine cells. 4. Yoshino. N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts. cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.

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Amount: 0.025 mg

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Delivery expected until 5/30/2024