Comparison

IGFBP2 Antibody (C-term)

Item no. ABC-AP8588B
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IF, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products IGFBP-2, IGFBP2, IBP2, IBP-2, Insulin-like growth factor-binding protein 2, IGF-binding protein 2, BP2
Available
Primary Accession
P18065
Antigen Type
Synthetic Peptide
Application
FC, IF, IHC-P, WB
Bio Background
IGFBP2-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Bio References
Arafat, A.M., et.al., J. Clin. Endocrinol. Metab. 94 (12), 5093-5101 (2009)
Yazawa, T., et.al., Am. J. Pathol. 175 (3), 976-987 (2009)
Clonality
Polyclonal
Clone/Animal Names
RB22493
Gene ID
3485
Gene Name
IGFBP2
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Confocal immunofluorescent analysis of IGFBP2 Antibody (C-term)(Cat. #AP8588b) with A549 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1
IF
Dilution image 1
1:10~50
Legend image 2
Anti-IGFBP2 Antibody (C-term) at 1:1000 dilution + T47D whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 35 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Formalin-fixed and paraffin-embedded human brain tissue with IGFBP2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3
IHC-P
Dilution image 3
1:10~50
Legend image 4
Flow cytometric analysis of jurkat cells using IGFBP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4
FC
Dilution image 4
1:10~50
Other Accession
P24853; P13384; Q29400
Predicted
B, Pig, Sh
Isotype
Rabbit Ig
Calculated MW
34814
Antigen Region
277-305
Antigen Source
HUMAN
Target/Specificity
This IGFBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-305 amino acids from the C-terminal region of human IGFBP2.
NCBI Accession
NP_000588.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
available

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