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Mebiol Gel

Mebiol Gel

Item no.

MBG-PMW20-1001

Manufacturer

Cosmobio

Amount

1 x 10 ml

Quantity options

1 x 10 ml 5 x 10 ml 20 x 10 ml 30 x 10 ml 40 x 10 ml 50 x 10 ml 1 x 50 ml 5 x 50 ml 20 x 50 ml

Category

Type

Reagents

Specific against

other

Conjugate/Tag

Unlabeled_

Citations

1. Yoshioka, H., Mikami, M., Mori, Y. and Tsuchida, E., ''A synthetic hydrogel with thermoreversible gelation.I.:Preparation and rheological properties.'', J. Macromol. Sci., A31(1), 113-120 (1994)
2. Yoshioka, H., Mikami, M., Mori, Y. and Tsuchida, E., ''Asynthetic hydrogel with thermoreversible gelation.II.:Effect of added salts.'', J. Macromol.Sci., A31(1), 121-125 (1994)
3. Yoshioka, H., Cushman, J.A., Mori, Y. and Tsuchida, E., ''A synthetic hydrogel with thermoreversible gelation. III. : anNMR studyof the sol-gel transition.'', Polym. Adv. Tech.,5, 122-127 (1994)
4. Shimizu, S., Yamazaki , M., Kubota, S., T. Ozasa, H. Moriya, Kobayashi, K., Mikami, M., Mori, Y., and Yamaguchi, S.,''In vitro studies on a new method for islet microencapsulationusing a thermoreversible gelation polymer, N-isopropylacrylamide -based copolymer'',Artifcial Organs, 20(11), 1232 (1996)
Yoshioka, H., Mori, Y., Tsukikawa, S. and Kubota, S., ''Thermoreversible gelation on heating and on cooling of an aqueousgelatin-poly(N-isopropylacrylamide) conjugate.'',Polym. Adv. Tech., 9, 155-158 (1998)
5. Tsukikawa, S., Matsuoka, H., Kurahashi, Y., Konno, Y., Satoh, K., Satoh, R., Isogai, A.,Kimura, K., Watanabe, Y., Nakano, S., Hayashi, J., Kubota, S.,''A new method to prepare multicellar spheroids in cancer cell lines using a thermo-reversible gelation polymer'', Artifcial Organs, 27(7), 598 -604(2003)
6. Yoshioka, H., Mori, Y. and Shimizu, M., ''Separation and recovery of DNA fragments by electrophoresisthrough a thermoreversible hydrogel composed of poly(ethylene oxide) and poly(propylene oxide).'', Analytical Biochemistry., 323 (2), 218-223 (2003)
7. Hishikawa, K., Miura, S., Marumo, T., Yoshioka, H., Mori, Y., Takato, T., Fujita, T., ''Gene expression profile ofhuman mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer'', Biochem. Biophys. Res. Commun.,317, 1103-1107 (2004).
8. H. N. Madhavan, J. Malathi, Patricia, Rinku Joseph, Yuichi Mori, Samuel JK Abraham and Hiroshi Yoshioka, ''A study on the growth of continuous culture cell lines embedded in Mebiol Gel'', CURRENT SCIENCE 87(9), 1275-1277 (2004)
9. M. Nagaya, S. Kubota, N. Suzuki, M. Tadokoro, K. Akashi, ''Evaluation of thermoreversiblegelation polymer for regeneration of focal liver injury'', Eur. Surg. Res.,36, 95-103 (2004)
T. Arai, T. Joki, M. Akiyama, M. Agawa, Y. Mori, H. Yoshioka, T. Abe, ''Novel drug delivery system using thermoreversible gelation polymer for malignant glioma'', J Neurooncol., 77,9-15 (2006)
Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita, T. Funatsu, ''On-Chip Cell Sorting System Using Laser-Induced Heating of a Thermoreversible GelationPolymer to Control Flow'', Anal Chem., 78,695-701(2006)
10. Reinhold J.Medina, Ken Kataoka, Mikiro Takaishi, Masahiro Miyazaki, Nam-ho Huh, ''Isolation of epithelial stem cells from dermis by a three dimensional culture system'', Journal of Cellular Biochemistry, 98(1), 174-184 (2006)
11. Nagaya, M., Kubota, S., Suzuki, N.,Akashi, K., Mitaka, T., ''Thermoreversible gelation polymer induces the emergence of hepatic stem cells in the partially injured rat liver'', Hepatology, 43, 1053-1062 (2006)
12. Yasuda, K. Kojima, K. W. Tinsley,H. Yoshioka, Y. Mori, C. A. Vacanti, ''In Vitro Culture of Chondrocytes in a Novel Thermoreversible Gelation Polymer Scaffold Containing Growth Factors'', Tissue Engineering,12(5), 1237-1245 (2006)
13. K. Murakami, K. Ishii, Y. Ishihara, S. Yoshizaki, K. Tanaka. Y. Gotoh, H. Aizaki, M. Kohara, H.Yoshioka, Y. Mori, N. Manabe, I., Shoji, T. Sata, R. Bartenschlarger, Y. Matsuura, T. Miyamura, T. Suzuki, ''Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b'', Virology, 351 (2), 381-392 (2006)
B. Sudha, H. N. Madhavan, G. Sitalakshmi, J. Malathi, S. Krishnakumar, Y. Mori, H. Yoshioka, S. Abraham, ''Cultivation of human corneal

ECLASS 10.1

42051003

ECLASS 11.0

42051003

UNSPSC

12000000

Available

Manufacturer - Category

Cells & Cell Culture/3D Cell Culture/Mebiol Gel

Maker

MBG

Short Description

Mebiol® Gel—a copolymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) (PNIPAAm-PEG)—undergoes temperature-reversible transition from a liquid-like state at 4°C to rigid hydrogel at 37°C. \\The highly lipophilic hydrogel state provides niches for cell proliferation, cell communication, gas/mass exchange, and shear force protection—ideal for 3D cell culture, tissue engineering, and drug delivery.

Description

Note:
This product is packaged with RP Drying Agent packs to absorb moisture, oxygen and corrosive gases. It is therefore subject to European Commerce Area Import Regulation (EC) No. 1272/2008 - Classification, Labeling, and Packaging of Substances and Mixtures (CLP). Applies to EEA (i.e. EU27 countries, Iceland, Liechtenstein, Norway, UK).
For additional information, see the following:
OSHA Europe

RP Agent Manufactuer's Statement

Introduction to Mebiol® Gel

Hydrogels are a diverse class of polymeric materials characterized by their network-like structure and high water content. Hydrogels of many kinds have found a wide variety of applications in medicine and life science research weighted towards, but not at all limited to three-dimensional cell culture, tissue engineering, and drug delivery. Properties highly favorable to cell culture and tissue engineering applications prompted the commercialization of Mebiol® Gel, a copolymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) (PNIPAAm-PEG) for research purposes in the early 2000's.
Mebiol® Gel's defining feature, in contrast to other commercially available hydrogels, is its temperature reversible sol-gel transition. When cooled, Mebiol® Gel is a sol (handles like a liquid) but becomes a rigid hydrogel at higher temperatures. In practice, this means extremely easy cell handling. Cultures are seeded into cooled Mebiol® Gel and recovered conveniently by cooling the culture vessel and centrifugation. In the gel state, the highly lipophylic environment of the Mebiol® Gel presents an efficient niche for cell proliferation, cell communication, gas and mass exchange, and protection of cells and tissue from shear forces.

Low Temperature (Sol)
CTLGNU	Mebiol##(R)### Gel
Manufacturer - Category 2	Cell Culture System and Assay System
Manufacturer - Category 3	Mebiol##(R)### Gel 3D (3D Culture Matrix In The 21St Century )

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.