EMD 66684

Non Antibodies

AT1R receptors

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

547, 05

An Antagonist of Angiotensin II Receptor Type-1

Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

41116134

EMD 66684 is an Angiotensin II Type 1 Receptor Antagonist1.

Yes

Angiotensin II is responsible for vasoconstriction in the renin-angiotensin system. In addition to its direct effect on blood vessels it stimulates the synthesis and release of aldosterone and also promotes renal tubular reabsorption of sodium, resulting in water retention1.EMD 66684 is a synthetic antagonist of the Angiotensin II type 1 receptor. It has an effective concentration of 1-100 nM. EMD 66684 completely attenuates Ang II-induced increases in basal and nerve stimulated neuropeptide Y (NPY) overflow from mesenteric arterial beds in a model of spontaneously hypertensive rats. In the same model EMD 66684 also attenuated the effect of Ang II on perfusion pressure2.The antagonistic properties of EMD are also demonstrated in an experiment examining the relation between lipid rafts and angiotensin II receptors. EMD reduces Ang II-induced proliferation of neonatal pig glomerular mesangial cells. EMD also inhibits Ang II-mediated Ca2+ currents and the signal pathway initiated by calcium/calmodulin dependent protein kinases (CAMK) in the same cells3.