sFTX-303

Non Antibodies

P-type Ca2+ channels

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

287, 4

A Blocker of P-Type CaV Channels

Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

41116134

sFTX-3.3, a synthetic funnel web spider toxin1-2, is a polyamine amide analogue of FTX. It blocks P-, N-, and L-type Ca2+ channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies3.

Yes

Polyamine amide spider toxins inhibit neuronal voltage-activated Ca2+ currents. A terminal arginine group is an important feature of these Ca2+ channel antagonists1-2.FTX is a polyamine component of the venom of the American funnel web spider Agelenopsis aperta, and has been described as a selective antagonist of P-type voltage-dependent Ca2+ channels3-4. However, it has been demonstrated that FTX has actions at other sites including NMDA and GABA receptors and also on T-type calcium channels5.sFTX-3.3, a synthetic funnel web spider toxin6-7, is a polyamine amide analogue of FTX. The structures of these polyamine containing compounds are not identical: sFTX-3.3 contains an amide carbonyl oxygen that is absent from the predicted structure of native FTX8. sFTX-3.3 has been shown to block P-type calcium channels in rat cerebellar Purkinje cells8 and antagonizes P-, N-, and L-type calcium channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies. Its IC50 against P-, N-, and L-type channels are ~0.24 mM, ~0.7 mM and ~0.7 mM, respectively9.