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alpha-Latrotoxin

alpha-Latrotoxin

Item no.	ALO-LSP-130-40ug
Manufacturer	Alomone
CASRN	65988-34-3
Amount	40 ug
Quantity options	0.2 mg 40 ug 5 x 40 ug
Category	Oncology Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite Reagents & Chemicals Molecules By Organ Lung Cancer
Type	Molecules
Format	Lyophilized
Specific against	other
Purity	≥98% (HPLC)
Formula	Lyopholized in 20mM KPO4 pH 6.8.
ECLASS 10.1	32169090
ECLASS 11.0	32169090
UNSPSC	12000000
Shipping Condition	Room temperature
Available
Manufacturer - Type	Non Antibodies
Manufacturer - Applications	Electrophysiology, Synaptic recording
Manufacturer - Category	Proteins
Manufacturer - Targets	Neurexin-1, ADGRL1 (latrophilin-1/CIRL), and tyrosine-protein phosphatase S (PTPRS) receptors
Country of Origin	Israel
Shipping Temperature	The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
Storage Conditions	Storage as Solution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. - Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. - Storage after Reconstitution: Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.
Molecular Weight	130 kDa
Manufacturer - Format	Lyophilized
Short description	An Inducer of Neurotransmitter Release
Description	α-Latrotoxin-Lt1a, α-LTX-Lt1a, α-LTX - An Inducer of Neurotransmitter Release
Reconstitution	Centrifuge the vial (10, 000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. Soluble in water. Once dissolved in water, add an equal volume of glycerol. Do not shake or vortex. Avoid multiple freeze-thaw cycles to maintain biological activity.
Specificity	An Inducer of Neurotransmitter Release
PH	7, 4
UNSPSC	12352202
Origin	Latrodectus tredecimguttatus (Mediterranean black widow spider).
Modifications	Highly disulfide bridged protein Glycosylated
Effective Concentration	150 pM - 1 nM
Activity	α-Latrotoxin induces exhaustive neurotransmitter release from nerve terminals1. Indirectly activates the ERK MAP kinase pathway.
Solubility	Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in water. Once dissolved in water, add an equal volume of glycerol. Do not shake or vortex. Avoid multiple freeze-thawing cycles.
Sterile endotoxin free	yes
Bioassay tested	yes
Scientific Background	α-Latrotoxin is a 130 kDa protein toxin from the black widow spider venom and is the only protein in the venom that affects mammals.1, 2 Application of the toxin to presynaptic preparations induces, after a delay, a huge increase in spontaneous neurotransmitter release, which can be evaluated by measuring the post synaptic response in the form of miniature end plate potentials. This toxin is widely used to induce and study neurotransmitter release, but the molecular mechanism of its action is not fully determined.α-Latrotoxin is isolated according to a modified protocol as described by Frontali3 and Grasso4.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ALO-LSP-130-40ug-safety-datasheet.pdf