NMDA

Non Antibodies

NMDA receptors

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

147, 1

An NMDA Receptor Agonist

Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity

41116134

Prototypic NMDA receptor agonist. An amino acid that, as a D-isomer, is the defining agonist for the NMDA receptor subtype of glutamate receptors1. NMDA is a water-soluble synthetic substance that is not normally found in biological tissues2. EC50 ranges between 6.5-25.0 μM, depending on the tissue applied on1, 3, 4.

Yes

NMDA is the prototypic NMDA receptor agonist. An amino acid that, as a D-isomer, is the defining agonist for the NMDA receptor subtype of glutamate receptors1.NMDA is a water-soluble synthetic substance that is not normally found in biological tissues. It was first synthesized in the 1960s. NMDA is an excitotoxin in high quantities (it kills nerve cells by over-exciting them)2.NMDA activates NMDA receptors by binding to NR2 subunits, leading to the opening of a non-specific cation channel, which can allow the passage of Ca2+ and Na+ ions into the cell and K+ ions out of the cell3.NMDA was evaluated for the inhibition of [3H]MK-801 binding at N-methyl-D-aspartate glutamate receptor and IC50 with an IC50 of 6.86 µM1. Cortical cultures of rat brain neurons have an estimated EC50 value of 6.8 µM4. The EC50 found for NR2 subunit expressed in Xenopus oocytes was 21.7 µM3.