PACA

Non Antibodies

Neurite outgrowth, Nrf2 pathway

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

217, 22

A Potentiator of NGF-Induced Neurite Outgrowth

Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

41116134

N-propargyl caffeate amide (PACA) potentiates 2 ng/ml NGF-induced neurite outgrowth in PC12 cells at 5-20 µM. PACA was also shown to reduce 6-OHDA-induced neurotoxicity in dopaminergic PC12 cells and primary rat midbrain neurons, possibly by activating the Nrf2/HO-1 pathway1.

Yes

N-propargyl caffeate amide (PACA) enhances nerve growth factor (NGF)-induced neurite outgrowth, it was also shown to attenuate 6-hydroxydopamine (6-OHDA) neurotoxicity in PC12 cells and primary rat midbrain neurons.PACA interacts with kelch-like ECH-associated protein 1 (Keap1) and these conjugates induce the nuclear translocation of transcription factor Nrf2 and the expression of antioxidant heme oxygenase-1 (HO-1), hence PACA exhibits neuroprotective and neuritogenic activities via the Nrf2/HO-1 antioxidant pathway1.NGF belongs to a protein family called Neurotrophins, members of this family can regulate adult nervous system plasticity by promoting neuronal survival and stimulating nerve regrowth following injury. Insufficient production of neurotrophic factors is implicated in the pathogenesis of various neurodegenerative disorders and hence these factors have the potential to be used as therapeutic agents2.Nerve growth factor (NGF) regulates the microtubule-dependent extension and maintenance of axons by peripheral neurons. It also promotes microtubule assembly during neurite outgrowth3. NGF participates in the viability, development and preservation of neurons in mammalian nervous systems4, 5. In addition to neuronal cells, NGF expression has been found in several types of glial cells, including olfactory cells, microglial cells, oligodendrocytes, and astrocytes6. Recent studies have shown that NGF leads to neuronal apoptosis in aging, and neurodegenerative distress, and it was also shown to have a role in Alzheimer's disease7.