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Maurocalcine

Maurocalcine

Item no.	ALO-RTM-100-1mg
Manufacturer	Alomone
CASRN	269745-22-4
Amount	1 mg
Quantity options	0.1 mg 0.25 mg 0.5 mg 10 mg 1 mg 5 mg
Category	Oncology By Cell Type Carcinoma Reagents & Chemicals Molecules By Organ Colorectal Cancer
Type	Molecules
Format	Lyophilized
Specific against	other
Purity	≥98% (HPLC)
Formula	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Sequence	GDCLPHLKLCKENKDCCSKKCKRRGTNIEKRCR-OH
ECLASS 10.1	32169090
ECLASS 11.0	32169090
UNSPSC	12000000
Shipping Condition	Room temperature
Available
Manufacturer - Type	Non Antibodies
Manufacturer - Applications	Calcium imaging assay
Manufacturer - Category	Proteins
Manufacturer - Targets	Ryanodine receptors
Country of Origin	Israel
Shipping Temperature	The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
Storage Conditions	Storage as Solution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. - Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Molecular Weight	3858.6 Da
Manufacturer - Format	Lyophilized
Short description	A Ryanodine Receptor 1, 2 & 3 Modulator
Description	MCa, Maurocalcin - A Ryanodine Receptor 1, 2 & 3 Modulator
Reconstitution	Centrifuge the vial (10, 000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Specificity	A Ryanodine Receptor 1, 2 & 3 Modulator
PH	7, 4
UNSPSC	12352202
Origin	Scorpio maurus palmatus (Israeli golden scorpion)
Modifications	Disulfide bonds between: Cys³-Cys¹⁷, Cys¹⁰-Cys²¹ and Cys¹⁶-Cys³²
Effective Concentration	200 - 400 nM
Activity	Maurocalcine is an agonist of Ryanodine receptor channel (RyR) types 1' 2 and 3.
Solubility	Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Sterile endotoxin free	no
Bioassay tested	yes
Scientific Background	Maurocalcine (MCa) is a peptidyl toxin originally isolated from the venom of the scorpion Scorpio maurus palmatus.Native MCa is present at a low concentration in the venom (0.5% of total protein). This 33-mer basic peptide contains three disulfide bridges and has 82% sequence homology to Imperatoxin A (IpTxa), a scorpion toxin from the venom of Pandinus imperator. MCa shares the highly basic structural domain identified in peptide A - a segment of the dihydropyridine receptor and IpTxa which is important for interactions with ryanodine receptors. MCa also appears to share the same features as the so-called cell-penetrating peptides which allows the toxin to efficiently penetrate various cell types without expending metabolic energy or using an endocytotic mechanisM1-4In vitro, electrophysiological experiments based on recordings of single channels incorporated into planar lipid bilayers showed that MCa potently and reversibly modifies channel gating behavior of the ryanodine receptor 1 (RyR1) by inducing prominent subconductance behavior.1, 2Similar to IpTxa, MCa-modified channels can reversibly shuttle between subconductance states and fast gating states. The actions of these peptides were also found to be additive with those of ryanodine, resulting in additional sub-states along with the ryanodine-locked half-conducting state. However, the predominant subconductance induced by MCa differs from that of IpTxa. At a holding potential of +40 mV, the predominant substates induced by sMCa and IpTxa are 48 and 28% of the full conductance, respectively, suggesting that the structural difference in these peptides may induce slightly different channel conformations that stabilize different unitary conductances.5

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ALO-RTM-100-1mg-safety-datasheet.pdf